Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations.
Lyve1
MHCII
aging
cell biology
flow cytometry
mouse
muscle-resident macrophages
single-cell analysis
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 10 2022
19 10 2022
Historique:
received:
17
02
2022
accepted:
10
10
2022
pubmed:
20
10
2022
medline:
5
11
2022
entrez:
19
10
2022
Statut:
epublish
Résumé
Tissue-resident macrophages represent a group of highly responsive innate immune cells that acquire diverse functions by polarizing toward distinct subpopulations. The subpopulations of macrophages that reside in skeletal muscle (SKM) and their changes during aging are poorly characterized. By single-cell transcriptomic analysis with unsupervised clustering, we found 11 distinct macrophage clusters in male mouse SKM with enriched gene expression programs linked to reparative, proinflammatory, phagocytic, proliferative, and senescence-associated functions. Using a complementary classification, membrane markers LYVE1 and MHCII identified four macrophage subgroups: LYVE1-/MHCII
Identifiants
pubmed: 36259488
doi: 10.7554/eLife.77974
pii: 77974
pmc: PMC9629833
doi:
pii:
Substances chimiques
Biomarkers
0
Banques de données
GEO
['GSE195507']
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Intramural NIH HHS
ID : Z01 AG000511
Pays : United States
Déclaration de conflit d'intérêts
LK, PC, CC, KM, CD, YP, JF, CS, TW, CN, IR, RM, DT, SD, PS, LF, MG No competing interests declared
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