Spatial Multiomics of Lipids, N-Glycans, and Tryptic Peptides on a Single FFPE Tissue Section.
MALDI-MS imaging
N-glycomics
lipidomics
multiomics
proteomics
renal cancer
spatial proteomics
tumor
Journal
Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775
Informations de publication
Date de publication:
04 11 2022
04 11 2022
Historique:
pubmed:
20
10
2022
medline:
8
11
2022
entrez:
19
10
2022
Statut:
ppublish
Résumé
Mass spectrometry imaging (MSI) is an emerging technology that is capable of mapping various biomolecules within their native spatial context, and performing spatial multiomics on formalin-fixed paraffin-embedded (FFPE) tissues may further increase the molecular characterization of pathological states. Here we present a novel workflow which enables the sequential MSI of lipids, N-glycans, and tryptic peptides on a single FFPE tissue section and highlight the enhanced molecular characterization that is offered by combining the multiple spatial omics data sets. In murine brain and clear cell renal cell carcinoma (ccRCC) tissue, the three molecular levels provided complementary information and characterized different histological regions. Moreover, when the spatial omics data was integrated, the different histopathological regions of the ccRCC tissue could be better discriminated with respect to the imaging data set of any single omics class. Taken together, these promising findings demonstrate the capability to more comprehensively map the molecular complexity within pathological tissue.
Identifiants
pubmed: 36259755
doi: 10.1021/acs.jproteome.2c00601
pmc: PMC9639202
doi:
Substances chimiques
Formaldehyde
1HG84L3525
Peptides
0
Polysaccharides
0
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2798-2809Commentaires et corrections
Type : ErratumIn
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