Two firing modes and well-resolved Na


Journal

Pflugers Archiv : European journal of physiology
ISSN: 1432-2013
Titre abrégé: Pflugers Arch
Pays: Germany
ID NLM: 0154720

Informations de publication

Date de publication:
02 2023
Historique:
received: 04 08 2022
accepted: 07 10 2022
revised: 23 09 2022
pubmed: 20 10 2022
medline: 21 1 2023
entrez: 19 10 2022
Statut: ppublish

Résumé

We recorded spontaneous extracellular action potentials (eAPs) from rat chromaffin cells (CCs) at 37 °C using microelectrode arrays (MEAs) and compared them with intracellularly recorded APs (iAPs) through conventional patch clamp recordings at 22 °C. We show the existence of two distinct firing modes on MEAs: a ~ 4 Hz irregular continuous firing and a frequent intermittent firing mode where periods of high-intraburst frequency (~ 8 Hz) of ~ 7 s duration are interrupted by silent periods of ~ 12 s. eAPs occurred either as negative- or positive-going signals depending on the contact between cell and microelectrode: either predominantly controlled by junction-membrane ion channels (negative-going) or capacitive/ohmic coupling (positive-going). Negative-going eAPs were found to represent the trajectory of the Na

Identifiants

pubmed: 36260174
doi: 10.1007/s00424-022-02761-0
pii: 10.1007/s00424-022-02761-0
pmc: PMC9849155
doi:

Substances chimiques

Large-Conductance Calcium-Activated Potassium Channels 0
Ion Channels 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-202

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2022. The Author(s).

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Auteurs

Andrea Marcantoni (A)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy. andrea.marcantoni@unito.it.

Giuseppe Chiantia (G)

Department of Neuroscience, University of Torino, 10125, Turin, Italy.

Giulia Tomagra (G)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy.

Enis Hidisoglu (E)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy.

Claudio Franchino (C)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy.

Valentina Carabelli (V)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy.

Emilio Carbone (E)

Department of Drug Science, Laboratory of Cell Physiology and Molecular Neuroscience, N.I.S. Centre, University of Torino, Corso Raffaello 30, 10125, Turin, Italy. emilio.carbone@unito.it.

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