Optimization, validation and application of a high-throughput 96-well elution protocol for the quantification of psychoactive substances in influent wastewater.
96-well solid-phase extraction
high-throughput analysis
psychoactive substances
wastewater-based epidemiology
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
revised:
06
10
2022
received:
14
09
2022
accepted:
17
10
2022
pubmed:
20
10
2022
medline:
14
2
2023
entrez:
19
10
2022
Statut:
ppublish
Résumé
Wastewater-based epidemiology (WBE) is based on the analysis of human metabolic excretion products (biomarkers) of xenobiotics in wastewater, to gain information about various lifestyles and health aspects of a population in an evidence-based manner. Due to the complex wastewater matrix and trace level occurrence of human biomarkers in the sewage network, it is crucial to have sensitive analytical procedures available. Additionally, to improve the value of WBE as a complementary epidemiological source, there is increasing pressure on the analysis of more compounds, more locations and more samples. A high-throughput method based on 96-well Oasis MCX solid-phase extraction (SPE), requiring less influent wastewater (2 mL), was developed in accordance with the European Medicines Agency guidelines. Validation was successful for 28 parent drugs and metabolites of antidepressants, opioids and drugs of abuse. The selection of biomarkers and quantification limit was chosen to be relevant for WBE and was predominantly 10 ng/L or below. The final method was successfully applied to 24-h composite samples of October 2019 (n = 27), obtained from an urban wastewater treatment plant in Leuven (Belgium).
Substances chimiques
Wastewater
0
Water Pollutants, Chemical
0
Antidepressive Agents
0
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
240-246Subventions
Organisme : European Union's Justice Programme-Drugs Policy Initiatives
ID : 861602
Organisme : Research Agency of Republic of Slovenia (ARRS)
ID : P1-0143
Organisme : Research Agency of Republic of Slovenia (ARRS)
ID : N1-0143
Organisme : Research Scientific Foundation Flanders
ID : G060920N
Informations de copyright
© 2022 John Wiley & Sons Ltd.
Références
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