Peripheral mononuclear cells composition in platelet-rich fibrin in canines with chronic conditions.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 10 2022
19 10 2022
Historique:
received:
20
04
2022
accepted:
14
10
2022
entrez:
19
10
2022
pubmed:
20
10
2022
medline:
22
10
2022
Statut:
epublish
Résumé
Platelet-rich fibrin (PRF) is a hot research topic because of its regenerative effect in humans. However, data reporting about its application in companion animals is lacking. The study aimed to supplement currently available data on PRF cell composition in canine patients by isolating peripheral blood mononuclear cells (PBMC), namely T cells, matured B cells, monocytes and macrophages, and adapting current protocols of cell flow cytometry for PRF analysis. The canine patient population was divided into three subgroups: animals with periodontitis only, animals with neoplasia and periodontitis, and healthy controls. Individual clinical parameters of the patients and evaluation of the wound healing quality were included in the research. In the present study, canine PRF cell composition was analyzed for the first-time using cell flow cytometry protocol. A higher proportion of PBMC cells related to wound healing (CD3+, CD3+ CD4+ CD8-, CD14+) were found in the PRF of control, periodontitis and neoplasia groups compared to the respective blood samples, which implies a positive outcome associated with clinical PRF usage in canine patients. Proportions of monocytes and macrophages were higher in PRF samples compared to the blood of healthy patients and periodontitis-affected patients. However, inflammatory and neoplastic processes do not affect the distribution of PBMC.
Identifiants
pubmed: 36261500
doi: 10.1038/s41598-022-22487-4
pii: 10.1038/s41598-022-22487-4
pmc: PMC9582024
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17426Informations de copyright
© 2022. The Author(s).
Références
Cytokine. 2021 Dec;148:155695
pubmed: 34496340
J Oral Implantol. 2014 Dec;40(6):679-89
pubmed: 24945603
Oral Surg Oral Med Oral Pathol Oral Radiol. 2019 Jan;127(1):8-18
pubmed: 30287202
Front Immunol. 2018 Mar 01;9:339
pubmed: 29545794
Nat Rev Immunol. 2008 Jul;8(7):512-22
pubmed: 18469829
Front Immunol. 2020 Feb 26;11:330
pubmed: 32210958
Am J Sports Med. 2011 Oct;39(10):2135-40
pubmed: 21846925
Int J Implant Dent. 2021 Dec 19;7(1):117
pubmed: 34923613
Saudi Dent J. 2012 Jan;24(1):17-21
pubmed: 23960523
Int J Vet Sci Med. 2019 Nov 18;7(1):43-54
pubmed: 31819890
Sci Immunol. 2020 May 1;5(47):
pubmed: 32358172
Ann Maxillofac Surg. 2018 Jan-Jun;8(1):28-34
pubmed: 29963421
Adv Wound Care (New Rochelle). 2019 Nov 1;8(11):527-537
pubmed: 31637099
Int J Mol Sci. 2022 Jan 16;23(2):
pubmed: 35055157
Wound Repair Regen. 2017 Sep;25(5):774-791
pubmed: 28922523
Cancer Med. 2020 Jul;9(14):5086-5094
pubmed: 32459060
Transfus Med Rev. 2010 Jul;24(3):218-34
pubmed: 20656189
J Clin Invest. 2011 Mar;121(3):985-97
pubmed: 21317534
Front Immunol. 2017 Dec 20;8:1866
pubmed: 29326724
Clin Oral Investig. 2021 Jul;25(7):4239-4249
pubmed: 33829349
Immunol Invest. 2018 Oct;47(7):643-653
pubmed: 29924680
Nat Immunol. 2013 May;14(5):509-13
pubmed: 23542740
Cytometry A. 2019 Feb;95(2):219-226
pubmed: 30523671
Acta Biomater. 2021 Oct 1;133:17-33
pubmed: 33905946
Arch Gynecol Obstet. 2022 Oct;306(4):1171-1176
pubmed: 35377044
J Indian Soc Periodontol. 2016 Mar-Apr;20(2):151-6
pubmed: 27143826
Vet Dermatol. 2022 Feb;33(1):91-e27
pubmed: 34704298
Clin Exp Dent Res. 2021 Aug;7(4):436-442
pubmed: 33443821
Metab Syndr Relat Disord. 2018 Sep;16(7):342-349
pubmed: 29957122
Arthroscopy. 2019 Oct;35(10):2878-2884.e1
pubmed: 31604507
J Biomed Mater Res A. 2020 Jan;108(1):61-68
pubmed: 31449340
Exp Hematol. 2021 Jan;93:1-13
pubmed: 33068648
J Immunother Cancer. 2019 Mar 28;7(1):85
pubmed: 30922400
Curr Dermatol Rep. 2018;7(4):350-358
pubmed: 30524911
Sci Rep. 2020 Feb 5;10(1):1850
pubmed: 32024893
Sci Rep. 2018 Jul 13;8(1):10642
pubmed: 30006555
J Clin Invest. 2004 Jun;113(11):1596-606
pubmed: 15173886
J Periodontal Implant Sci. 2018 Oct 24;48(5):274-283
pubmed: 30405935
J Tissue Eng Regen Med. 2018 Jan;12(1):30-43
pubmed: 27863082