Human herpes virus type-6 is associated with central nervous system infections in children in Sudan.
HHV-6
aseptic meningitis
real-time PCR
viral meningitis
viral neuroinfections
Journal
African journal of laboratory medicine
ISSN: 2225-2002
Titre abrégé: Afr J Lab Med
Pays: South Africa
ID NLM: 101603205
Informations de publication
Date de publication:
2022
2022
Historique:
received:
29
08
2021
accepted:
25
05
2022
entrez:
20
10
2022
pubmed:
21
10
2022
medline:
21
10
2022
Statut:
epublish
Résumé
Human herpes virus type-6 (HHV-6) is increasingly recognised as a febrile agent in children. However, less is known in sub-Saharan African countries, including Sudan. We investigated the involvement of HHV-6 in paediatric central nervous system (CNS) infections in Khartoum, Sudan. Febrile patients aged up to 15 years with suspected CNS infections at Omdurman Hospital for Children from 01 December 2009 to 01 August 2010 were included. Viral DNA was extracted from leftover cerebrospinal fluid (CSF) specimens and quantitatively amplified by real-time polymerase chain reaction (PCR) at Umeå University in Sweden. Of 503 CSF specimens, 13 (2.6%) were positive for HHV-6 (33.0% [13/40 of cases with proven infectious meningitis]). The median thermal cycle threshold for all HHV-6-positive specimens was 38 (range: 31.9-40.8). The median number of virus copies was 281.3/PCR run (1 × 10 Among children in Sudan with CNS infections, HHV-6 is common. Studies on the existence and spread of HHV-6 chromosomal integration in this population are needed.
Sections du résumé
Background
UNASSIGNED
Human herpes virus type-6 (HHV-6) is increasingly recognised as a febrile agent in children. However, less is known in sub-Saharan African countries, including Sudan.
Objective
UNASSIGNED
We investigated the involvement of HHV-6 in paediatric central nervous system (CNS) infections in Khartoum, Sudan.
Methods
UNASSIGNED
Febrile patients aged up to 15 years with suspected CNS infections at Omdurman Hospital for Children from 01 December 2009 to 01 August 2010 were included. Viral DNA was extracted from leftover cerebrospinal fluid (CSF) specimens and quantitatively amplified by real-time polymerase chain reaction (PCR) at Umeå University in Sweden.
Results
UNASSIGNED
Of 503 CSF specimens, 13 (2.6%) were positive for HHV-6 (33.0% [13/40 of cases with proven infectious meningitis]). The median thermal cycle threshold for all HHV-6-positive specimens was 38 (range: 31.9-40.8). The median number of virus copies was 281.3/PCR run (1 × 10
Conclusion
UNASSIGNED
Among children in Sudan with CNS infections, HHV-6 is common. Studies on the existence and spread of HHV-6 chromosomal integration in this population are needed.
Identifiants
pubmed: 36263389
doi: 10.4102/ajlm.v11i1.1718
pii: AJLM-11-1718
pmc: PMC9575351
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1718Informations de copyright
© 2022. The Authors.
Déclaration de conflit d'intérêts
We, the authors, declare that we have no competing interests with respect to the research, authorship or publication of this article.
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