Streptococcus mutans dexA affects exopolysaccharides production and biofilm homeostasis.
Streptococcus mutans
dexA
exopolysaccharides
multispecies biofilm
probiotics
Journal
Molecular oral microbiology
ISSN: 2041-1014
Titre abrégé: Mol Oral Microbiol
Pays: Denmark
ID NLM: 101524770
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
25
09
2022
received:
12
04
2022
accepted:
12
10
2022
pubmed:
22
10
2022
medline:
22
3
2023
entrez:
21
10
2022
Statut:
ppublish
Résumé
The study aimed to evaluate the role of Streptococcus mutans (S. mutans) dexA gene on biofilm structure and microecological distribution in multispecies biofilms. A multispecies biofilm model consisting of S. mutans and its dexA mutants, Streptococcus gordonii (S. gordonii) and Streptococcus sanguinis (S. sanguinis) was constructed, and bacterial growth, biofilm architecture and microbiota composition were determined to study the effect of the S. mutans dexA on multispecies biofilms. Our results showed that either deletion or overexpression of S. mutans dexA had no effect on the planktonic growth of bacterium, while S. mutans dominated in the multispecies biofilms to form cariogenic biofilms. Furthermore, we revealed that the SmudexA+ group showed structural abnormality in the form of more fractures and blank areas. The morphology of the SmudexA group was sparser and more porous, with reduced and less agglomerated exopolysaccharides scaffold. Interestingly, the microbiota composition analysis provided new insights that the inhibition of S. gordonii and S. sanguinis was alleviated in the SmudexA group compared to the significantly suppressed condition in the other groups. In conclusion, deletion of S. mutans dexA gene re-modules biofilm structure and microbiota composition, thereby leading to decreased cariogenicity. Thus, the S. mutans dexA may be an important target for regulating the cariogenicity of dental plaque biofilms, expecting to be a probiotic for caries control.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
134-144Subventions
Organisme : Hunan Provincial Natural Science Foundation of China
ID : 2020JJ4459
Organisme : Hunan Provincial Natural Science Foundation of China
ID : 2017JJ3403
Organisme : National Natural Science Foundation of China
ID : 81702709
Organisme : West China Hospital of Stomatology, Research and Develop Program
ID : RD-02-202001
Organisme : Central South University Education and Teaching Reform Project
ID : 2020JGB116
Organisme : Central South University Education and Teaching Reform Project
ID : 2020jy137-2
Informations de copyright
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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