Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells.

aging anisotropy endothelial cells monolayer integrity proliferation senescence telomere topography

Journal

Frontiers in cardiovascular medicine
ISSN: 2297-055X
Titre abrégé: Front Cardiovasc Med
Pays: Switzerland
ID NLM: 101653388

Informations de publication

Date de publication:
2022
Historique:
received: 26 05 2022
accepted: 31 08 2022
entrez: 24 10 2022
pubmed: 25 10 2022
medline: 25 10 2022
Statut: epublish

Résumé

Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs.

Identifiants

DOI: 10.3389/fcvm.2022.953582 PMID: 36277782 PMC: PMC9579341
pubmed: 36277782
doi: 10.3389/fcvm.2022.953582
pmc: PMC9579341
doi:

Types de publication

Journal Article

Langues

eng

Pagination

953582

Informations de copyright

Copyright © 2022 Exarchos, Neuber, Meyborg, Giampietro, Chala, Moimas, Hinkov, Kaufmann, Pramotton, Krüger, Rodriguez Cetina Biefer, Cesarovic, Poulikakos, Falk, Emmert, Ferrari and Nazari-Shafti.

Déclaration de conflit d'intérêts

Outside the submitted work: VF has relevant (institutional) financial activities with following commercial entities: Medtronic GmbH, Biotronik SE & Co., Abbott GmbH & Co. KG, Boston Scientific, Edwards Lifesciences, Berlin Heart, Novartis Pharma GmbH, JOTEC GmbH and Zurich Heart in relation to educational grants (including travel support), fees for lectures and speeches, fees for professional consultation and research and study funds. ME was a shareholder at LifeMatrix Technologies AG. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Vasileios Exarchos (V)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Sebastian Neuber (S)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Heike Meyborg (H)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Costanza Giampietro (C)

Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland.
Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland.

Nafsika Chala (N)

Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland.

Silvia Moimas (S)

Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland.

Hristian Hinkov (H)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Friedrich Kaufmann (F)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.

Francesca M Pramotton (FM)

Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland.
Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland.

Katrin Krüger (K)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.
Clinic for Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Hector Rodriguez Cetina Biefer (H)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.
Department of Cardiac Surgery, City Hospital of Zürich, Site Triemli, Zurich, Switzerland.

Nikola Cesarovic (N)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland.

Dimos Poulikakos (D)

Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland.

Volkmar Falk (V)

Clinic for Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland.
Department for Cardiovascular and Thoracic Surgery, German Heart Center Berlin, Berlin, Germany.

Maximilian Y Emmert (MY)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.
Clinic for Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Department of Health Sciences and Technology, ETH Zürich, Zurich, Switzerland.
Institute for Regenerative Medicine, University of Zurich, Zurich, Switzerland.

Aldo Ferrari (A)

Experimental Continuum Mechanics, Empa Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland.
Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zurich, Switzerland.
Laboratory of Thermodynamics in Emerging Technologies, Department of Mechanical and Process Engineering, ETH Zürich, Zurich, Switzerland.

Timo Z Nazari-Shafti (TZ)

Cardiosurgical Research Group, Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, Berlin, Germany.
Translational Cardiovascular Regenerative Technologies Group, BIH Center for Regenerative Therapies, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.
BIH Biomedical Innovation Academy, BIH Charité (Junior) (Digital) Clinician Scientist Program, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Classifications MeSH