Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
10
08
2021
accepted:
02
09
2022
pubmed:
26
10
2022
medline:
1
11
2022
entrez:
25
10
2022
Statut:
ppublish
Résumé
Interactions between respiratory viruses during infection affect transmission dynamics and clinical outcomes. To identify and characterize virus-virus interactions at the cellular level, we coinfected human lung cells with influenza A virus (IAV) and respiratory syncytial virus (RSV). Super-resolution microscopy, live-cell imaging, scanning electron microscopy and cryo-electron tomography revealed extracellular and membrane-associated filamentous structures consistent with hybrid viral particles (HVPs). We found that HVPs harbour surface glycoproteins and ribonucleoproteins of IAV and RSV. HVPs use the RSV fusion glycoprotein to evade anti-IAV neutralizing antibodies and infect and spread among cells lacking IAV receptors. Finally, we show that IAV and RSV coinfection in primary cells of the bronchial epithelium results in viral proteins from both viruses co-localizing at the apical cell surface. Our observations define a previously unknown interaction between respiratory viruses that might affect virus pathogenesis by expanding virus tropism and enabling immune evasion.
Identifiants
pubmed: 36280786
doi: 10.1038/s41564-022-01242-5
pii: 10.1038/s41564-022-01242-5
doi:
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1879-1890Subventions
Organisme : Medical Research Council
ID : MR/N013166/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/7
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_17135
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/9
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R502327/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Limited.
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