Inhibition of CGRP signaling impairs fracture healing in mice.
calcitonin gene-related peptide
fracture healing
murine model
nociception
periosteum
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
revised:
06
09
2022
received:
30
05
2022
accepted:
18
10
2022
pmc-release:
01
06
2024
medline:
8
5
2023
pubmed:
26
10
2022
entrez:
25
10
2022
Statut:
ppublish
Résumé
Calcitonin gene-related peptide (CGRP) is a neuropeptide produced by sensory nerves and functions as a pain sensor. It acts by binding to the calcitonin-like receptor (CLR, protein; Calcrl, gene). CGRP inhibition has been recently introduced as therapeutic treatment of migraine-associated pain. Previous studies have shown that CGRP stimulates bone formation. The aim of our study is to determine whether the inhibition of CGRP signaling negatively impacted fracture healing. Using α-smooth muscle actin (αSMA) Cre animals crossed with Ai9 reporter mice, we showed that CGRP-expressing nerves are near αSMA + cells in the periosteum. In vitro experiments revealed that periosteal cells express Calcrl and receptor activity modifying protein 1; and CGRP stimulation increased periosteal cell proliferation. Using a tamoxifen-inducible model αSMACre/CLR
Identifiants
pubmed: 36281531
doi: 10.1002/jor.25474
pmc: PMC10123175
mid: NIHMS1844888
doi:
Substances chimiques
Calcitonin Gene-Related Peptide
JHB2QIZ69Z
Calcitonin
9007-12-9
Receptors, Calcitonin Gene-Related Peptide
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1228-1239Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR055607
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070813
Pays : United States
Informations de copyright
© 2022 Orthopaedic Research Society. Published by Wiley Periodicals LLC.
Références
Curr Osteoporos Rep. 2015 Oct;13(5):327-35
pubmed: 26254939
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
J Orthop Res. 2019 Jan;37(1):35-50
pubmed: 30370699
Endocrinology. 2012 Apr;153(4):1850-60
pubmed: 22315449
Mech Ageing Dev. 2020 Jul;189:111265
pubmed: 32446790
Stem Cells. 2020 Apr;38(4):530-541
pubmed: 31859429
J Bone Miner Res. 2014;29(5):1283-94
pubmed: 24190076
Neuroscience. 2002;113(1):155-66
pubmed: 12123694
PLoS One. 2019 Sep 26;14(9):e0222602
pubmed: 31557169
Circulation. 1988 May;77(5):1072-80
pubmed: 2452035
Proc Natl Acad Sci U S A. 2017 May 2;114(18):E3632-E3641
pubmed: 28416686
Cell Rep. 2016 Sep 6;16(10):2723-2735
pubmed: 27568565
Elife. 2021 Feb 09;10:
pubmed: 33560227
JBMR Plus. 2019 Jan 15;3(5):e10127
pubmed: 31131345
Calcif Tissue Int. 1987 Mar;40(3):149-54
pubmed: 3105845
J Clin Invest. 2019 Dec 2;129(12):5137-5150
pubmed: 31638597
Nature. 1998 May 28;393(6683):333-9
pubmed: 9620797
J Bone Miner Res. 2021 May;36(5):1012-1025
pubmed: 33592122
Neuropeptides. 2019 Feb;73:78-88
pubmed: 30522780
Nature. 1984 Apr 12-18;308(5960):653-5
pubmed: 6369148
J Bone Miner Res. 1999 Aug;14(8):1302-9
pubmed: 10457262
J Biol Chem. 2000 Oct 6;275(40):31438-43
pubmed: 10903324
Digestion. 2002;66(4):197-203
pubmed: 12592095
Bone. 2021 Sep;150:116019
pubmed: 34023542
J Bone Miner Res. 1999 Jul;14(7):1067-74
pubmed: 10404006
Bone Joint Res. 2019 Jul 05;8(6):255-265
pubmed: 31346454
Nat Med. 2016 Oct;22(10):1160-1169
pubmed: 27571347
J Orthop Res. 2020 Nov;38(11):2350-2361
pubmed: 32141629
Nature. 1988 Sep 8;335(6186):164-7
pubmed: 2901042
Nature. 2021 Jan;589(7843):591-596
pubmed: 33361809
BMJ Open. 2013 Feb 08;3(2):
pubmed: 23396560
Physiol Rev. 2014 Oct;94(4):1099-142
pubmed: 25287861
Neuroscience. 2011 Mar 31;178:196-207
pubmed: 21277945
EBioMedicine. 2020 Sep;59:102970
pubmed: 32853990
Regul Pept. 1985 Mar;10(2-3):189-97
pubmed: 3922013
Stroke. 1993 Jul;24(7):1043-7; discussion 1047-8
pubmed: 7686695
Bone. 2020 Feb;131:115109
pubmed: 31715336
Physiol Rev. 2019 Jan 1;99(1):781-805
pubmed: 30540227
Diabetologia. 2015 Mar;58(3):633-642
pubmed: 25563724
Curr Osteoporos Rep. 2017 Dec;15(6):601-608
pubmed: 29143915
PLoS One. 2011;6(6):e20386
pubmed: 21694766
J Immunol. 2011 Jun 15;186(12):6886-93
pubmed: 21551361
Peptides. 1990 Jul-Aug;11(4):625-32
pubmed: 2235673
Sci Adv. 2022 Mar 18;8(11):eabl5716
pubmed: 35302859
Biochim Biophys Acta Biomembr. 2020 Mar 1;1862(3):183174
pubmed: 31887275
J Neurochem. 2004 Dec;91(6):1417-29
pubmed: 15584918
J Clin Invest. 2008 Jan;118(1):40-50
pubmed: 18097475
Neuropeptides. 2003 Oct;37(5):316-8
pubmed: 14607109
Front Mol Neurosci. 2017 Sep 06;10:284
pubmed: 28932184
J Bone Miner Res. 2004 Dec;19(12):2049-56
pubmed: 15537449
Int Immunol. 2004 May;16(5):643-53
pubmed: 15096485
Curr Protein Pept Sci. 2013 Aug;14(5):407-15
pubmed: 23745704
Stem Cells. 2012 Feb;30(2):187-96
pubmed: 22083974