Cell-specific bioorthogonal tagging of glycoproteins.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 10 2022
25 10 2022
Historique:
received:
20
07
2022
accepted:
05
10
2022
entrez:
25
10
2022
pubmed:
26
10
2022
medline:
28
10
2022
Statut:
epublish
Résumé
Altered glycoprotein expression is an undisputed corollary of cancer development. Understanding these alterations is paramount but hampered by limitations underlying cellular model systems. For instance, the intricate interactions between tumour and host cannot be adequately recapitulated in monoculture of tumour-derived cell lines. More complex co-culture models usually rely on sorting procedures for proteome analyses and rarely capture the details of protein glycosylation. Here, we report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway that transforms bioorthogonally tagged sugars into the corresponding nucleotide-sugars. Only transfected cells incorporate bioorthogonal tags into glycoproteins in the presence of non-transfected cells. We employ BOCTAG as an imaging technique and to annotate cell-specific glycosylation sites in mass spectrometry-glycoproteomics. We demonstrate application in co-culture and mouse models, allowing for profiling of the glycoproteome as an important modulator of cellular function.
Identifiants
pubmed: 36284108
doi: 10.1038/s41467-022-33854-0
pii: 10.1038/s41467-022-33854-0
pmc: PMC9596482
doi:
Substances chimiques
Proteome
0
Glycoproteins
0
Sugars
0
Nucleotides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6237Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : EP/S005226/1
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : R01 CA200423
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM147039
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T01279X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M028836/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M02847X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M027791/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : EP/S013741/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 218304/Z/19/Z
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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