Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility.
ELISA
ZEB1
ZEB2
endometriosis
epithelial–mesenchymal transition
infertility
peritoneal fluid
plasma
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
01 Oct 2022
01 Oct 2022
Historique:
received:
14
08
2022
revised:
27
09
2022
accepted:
29
09
2022
entrez:
27
10
2022
pubmed:
28
10
2022
medline:
28
10
2022
Statut:
epublish
Résumé
Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial−mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 ± 12.70 ng/L vs. 12.07 ± 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes.
Identifiants
pubmed: 36289723
pii: biomedicines10102460
doi: 10.3390/biomedicines10102460
pmc: PMC9599446
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Polish Ministry of Health
ID : 6/6/4/1/NPZ/2017/1210/13522
Organisme : Medical University of Warsaw
ID : 1W51/2/M/MB/N/20
Organisme : the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement
ID : No. 101008193 TRENDO
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