An Optimized Workflow for the Discovery of New Antimicrobial Compounds Targeting Bacterial RNA Polymerase Complex Formation.
RNAP holoenzyme assembly
antibiotics
bacterial RNA polymerase (RNAP)
bacterial transcription inhibitors
drug-discovery workflow
protein–protein interaction (PPI)
protein–protein interaction inhibitor
yeast Bioluminescence Resonance Energy Transfer (yBRET)
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
21 Oct 2022
21 Oct 2022
Historique:
received:
29
09
2022
revised:
14
10
2022
accepted:
17
10
2022
entrez:
27
10
2022
pubmed:
28
10
2022
medline:
28
10
2022
Statut:
epublish
Résumé
Bacterial resistance represents a major health problem worldwide and there is an urgent need to develop first-in-class compounds directed against new therapeutic targets. We previously developed a drug-discovery platform to identify new antimicrobials able to disrupt the protein-protein interaction between the β' subunit and the σ
Identifiants
pubmed: 36290107
pii: antibiotics11101449
doi: 10.3390/antibiotics11101449
pmc: PMC9598883
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Italian Ministry of Education, University and Research
ID : Progetti Competitivi MUR ex DM 1059/2021
Organisme : University of Parma, Italy
ID : Bando Straordinario 591 di Ateneo per Progetti di Ricerca Biomedica in Ambito SARS-COV-2 e COVID-19- 2020
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