The Probiotic Strains Bifidοbacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii Regulate Wound Healing and Chemokine Responses in Human Intestinal Subepithelial Myofibroblasts
chemokines
human intestinal subepithelial myofibroblasts
probiotics
wound healing
Journal
Pharmaceuticals (Basel, Switzerland)
ISSN: 1424-8247
Titre abrégé: Pharmaceuticals (Basel)
Pays: Switzerland
ID NLM: 101238453
Informations de publication
Date de publication:
20 Oct 2022
20 Oct 2022
Historique:
received:
02
08
2022
revised:
07
10
2022
accepted:
18
10
2022
entrez:
27
10
2022
pubmed:
28
10
2022
medline:
28
10
2022
Statut:
epublish
Résumé
Bifidobacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii are common probiotic supplements. Colonic subepithelial myofibroblasts (cSEMFs) are actively involved in mucosal wound healing and inflammation. cSEMFs, isolated from healthy individuals, were stimulated with 102 or 104 cfu/mL of these probiotic strains alone and in combination, and their effect on chemokine and wound healing factor expression was assessed by qRT-PCR, ELISA and Sircol Assay, and on cSEMFs migration, by Wound Healing Assay. These strains remained viable and altered cSEMFs’ inflammatory and wound healing behavior, depending on the strain and concentration. cSEMFs treated with a combination of the four probiotics had a moderate, but statistically significant, increase in the mRNA and/or protein expression of chemokines CXCL1, CXCL2, CXCL4, CXCL8, CXCL10, CCL2 and CCL5, and healing factors, collagen type I and III, fibronectin and tissue factor. In contrast, when each strain was administered alone, different effects were observed, with greater increase or decrease in chemokine and healing factor expression, which was balanced by the mixture. Overall, this study highlights that the use of multiple probiotic strains can potentially alert the gut mucosal immune system and promote wound healing, having a better effect on mucosal immunity than the use of single probiotics.
Identifiants
pubmed: 36297405
pii: ph15101293
doi: 10.3390/ph15101293
pmc: PMC9611312
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : "AMKE KLEON TSETIS" Foundation, Athens, Greece
Organisme : IMPReS (MIS 5047189), financially supported by the Program "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020), co-financed by Greece and the European Union (European Regional Development Fund)
ID : MIS 5047189
Organisme : «Strategic expansion of the Greek Biobanking Infrastructure» (BBMRI-GR, NSRF 2014-2020)
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