Discovery and multi-parametric optimization of a high-affinity antibody against interleukin-25 with neutralizing activity in a mouse model of skin inflammation.

antibody engineering interleukin-25 psoriasis skin inflammation

Journal

Antibody therapeutics
ISSN: 2516-4236
Titre abrégé: Antib Ther
Pays: United States
ID NLM: 101730822

Informations de publication

Date de publication:
Oct 2022
Historique:
received: 06 07 2022
revised: 31 08 2022
accepted: 18 09 2022
entrez: 27 10 2022
pubmed: 28 10 2022
medline: 28 10 2022
Statut: epublish

Résumé

Interleukin (IL)25 has been implicated in tissue homeostasis at barrier surfaces and the initiation of type two inflammatory signaling in response to infection and cell injury across multiple organs. We sought to discover and engineer a high affinity neutralizing antibody and evaluate the antibody functional activity In this study, we generated a novel anti-IL25 antibody (22C7) and investigated the antibody's therapeutic potential for targeting IL25 in inflammation. A novel anti-IL25 antibody (22C7) was generated with equivalent The generation of 22C7, an anti-IL25 antibody with efficacy in a preclinical model of skin inflammation, raises the therapeutic potential for 22C7 use in the spectrum of IL25-mediated diseases.

Sections du résumé

Background UNASSIGNED
Interleukin (IL)25 has been implicated in tissue homeostasis at barrier surfaces and the initiation of type two inflammatory signaling in response to infection and cell injury across multiple organs. We sought to discover and engineer a high affinity neutralizing antibody and evaluate the antibody functional activity
Methods UNASSIGNED
In this study, we generated a novel anti-IL25 antibody (22C7) and investigated the antibody's therapeutic potential for targeting IL25 in inflammation.
Results UNASSIGNED
A novel anti-IL25 antibody (22C7) was generated with equivalent
Conclusions UNASSIGNED
The generation of 22C7, an anti-IL25 antibody with efficacy in a preclinical model of skin inflammation, raises the therapeutic potential for 22C7 use in the spectrum of IL25-mediated diseases.

Identifiants

pubmed: 36299415
doi: 10.1093/abt/tbac022
pii: tbac022
pmc: PMC9590316
doi:

Types de publication

Journal Article

Langues

eng

Pagination

258-267

Informations de copyright

© The Author(s) 2022. Published by Oxford University Press on behalf of Antibody Therapeutics. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

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Auteurs

Ruth Bone (R)

BioMedicine Design, Pfizer, Grange Castle Business Park, Clondalkin, Dublin 22, Ireland.

Brian J Fennell (BJ)

BioMedicine Design, Pfizer, Grange Castle Business Park, Clondalkin, Dublin 22, Ireland.

Amy Tam (A)

BioMedicine Design, Pfizer, Cambridge, MA, 02139, USA.

Richard Sheldon (R)

Inflammation & Immunology, Pfizer, Cambridge, MA, 02139, USA.

Karl Nocka (K)

Inflammation & Immunology, Pfizer, Cambridge, MA, 02139, USA.

Sreeja Varghese (S)

BioMedicine Design, Pfizer, Grange Castle Business Park, Clondalkin, Dublin 22, Ireland.

Chew Shun Chang (CS)

BioMedicine Design, Pfizer, Cambridge, MA, 02139, USA.

Heike C Hawerkamp (HC)

Trinity Biomedical Sciences Institute, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.

Aoife Yeow (A)

Trinity Biomedical Sciences Institute, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.

Sean P Saunders (SP)

Trinity Biomedical Sciences Institute, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.

Emily Hams (E)

Trinity Biomedical Sciences Institute, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.

Patrick T Walsh (PT)

National Children's Research Centre, Our Lady's Children's Hospital, Dublin 12, Ireland.
Trinity Translational Medicine Institute, Trinity College Dublin, St James's Hospital, Dublin 8, Ireland.

Orla Cunningham (O)

UltraHuman Eight Limited, C/o Kreston Reeves Llp, Kent, CT139FF, UK.

Padraic G Fallon (PG)

Trinity Biomedical Sciences Institute, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.
Trinity Translational Medicine Institute, Trinity College Dublin, St James's Hospital, Dublin 8, Ireland.

Classifications MeSH