Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.
Journal
Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676
Informations de publication
Date de publication:
08 Jun 2022
08 Jun 2022
Historique:
received:
09
02
2022
accepted:
24
03
2022
entrez:
27
10
2022
pubmed:
28
10
2022
medline:
28
10
2022
Statut:
epublish
Résumé
Cellular functions rely on proper actions of organelles such as peroxisomes. These organelles rely on the import of proteins from the cytosol. The peroxisomal import receptor PEX5 takes up target proteins in the cytosol and transports them to the peroxisomal matrix. However, its cytosolic molecular interactions have so far not directly been disclosed. Here, we combined advanced optical microscopy and spectroscopy techniques such as fluorescence correlation spectroscopy and stimulated emission depletion microscopy with biochemical tools to present a detailed characterization of the cytosolic diffusion and interaction dynamics of PEX5. Among other features, we highlight a slow diffusion of PEX5, independent of aggregation or target binding, but associated with cytosolic interaction partners via its N-terminal domain. This sheds new light on the functionality of the receptor in the cytosol as well as highlighting the potential of using complementary microscopy tools to decipher molecular interactions in the cytosol by studying their diffusion dynamics.
Identifiants
pubmed: 36299769
doi: 10.1016/j.bpr.2022.100055
pii: S2667-0747(22)00012-X
pmc: PMC9586885
doi:
Types de publication
Journal Article
Langues
eng
Pagination
NoneSubventions
Organisme : Medical Research Council
ID : MC_UU_00008/9
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12010/9
Pays : United Kingdom
Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Biochim Biophys Acta. 2006 Dec;1763(12):1605-12
pubmed: 17028014
Annu Rev Biophys Biomol Struct. 2005;34:351-78
pubmed: 15869394
Biochem Biophys Res Commun. 1999 Aug 27;262(2):504-8
pubmed: 10462504
Cytometry. 1999 Jul 1;36(3):176-82
pubmed: 10404965
Biophys J. 1996 Sep;71(3):1364-73
pubmed: 8874012
Mol Cell. 2006 Dec 8;24(5):653-663
pubmed: 17157249
Biophys J. 1997 Aug;73(2):1073-80
pubmed: 9251823
Nat Commun. 2017 Jul 6;8(1):65
pubmed: 28684735
Nat Methods. 2007 Nov;4(11):963-73
pubmed: 17971781
J Cell Biol. 1996 Dec;135(6 Pt 2):1763-74
pubmed: 8991089
J Biol Chem. 2009 Sep 11;284(37):25334-42
pubmed: 19584060
Opt Express. 2019 Aug 5;27(16):23378-23395
pubmed: 31510616
Nat Rev Mol Cell Biol. 2002 May;3(5):382-9
pubmed: 11988772
Methods. 2015 Oct 15;88:67-75
pubmed: 26123184
J Phys D Appl Phys. 2020 Apr 15;53(16):164003
pubmed: 33191951
Mol Biochem Parasitol. 1999 Oct 25;104(1):106-19
pubmed: 10589985
Genome Biol. 2016 Jul 05;17(1):148
pubmed: 27380939
Methods. 2018 May 1;140-141:3-9
pubmed: 29155128
iScience. 2020 Dec 04;24(1):101891
pubmed: 33364580
Methods Protoc. 2020 Jul 29;3(3):
pubmed: 32751356
Biophys J. 2003 May;84(5):3353-63
pubmed: 12719264
J Biol Chem. 2016 Aug 12;291(33):16948-62
pubmed: 27311714
Biophys J. 2001 Jun;80(6):2954-67
pubmed: 11371468
EMBO J. 2006 Jul 26;25(14):3245-56
pubmed: 16858413
J Cell Biol. 1994 Dec;127(5):1245-57
pubmed: 7962087
BMC Res Notes. 2009 Dec 10;2:243
pubmed: 20003239
J Cell Biol. 1999 Jan 25;144(2):255-66
pubmed: 9922452
J Biol Chem. 2014 Jan 3;289(1):437-48
pubmed: 24235149
Bioinformatics. 2016 Mar 15;32(6):958-60
pubmed: 26589275
J Biol Chem. 1999 Feb 26;274(9):5666-73
pubmed: 10026185
Nat Protoc. 2012 May 03;7(6):1042-51
pubmed: 22555243
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10541-5
pubmed: 7937990
Nat Cell Biol. 2010 Mar;12(3):273-7
pubmed: 20154681
J Biol Chem. 2001 Sep 14;276(37):34524-9
pubmed: 11438541
Nat Commun. 2020 Jan 29;11(1):578
pubmed: 31996685
Biophys J. 2018 May 22;114(10):2419-2431
pubmed: 29706225
BMC Biotechnol. 2011 Oct 12;11:92
pubmed: 21992524
Cell. 1987 Jan 16;48(1):165-73
pubmed: 3539364
Nat Protoc. 2015 May;10(5):660-80
pubmed: 25837418
Biochem J. 2001 Jul 1;357(Pt 1):157-65
pubmed: 11415446
ACS Nano. 2018 Aug 28;12(8):8540-8546
pubmed: 30028588
Mol Cell Biol. 1999 Mar;19(3):2265-77
pubmed: 10022913
Cell. 2016 Aug 11;166(4):920-934
pubmed: 27499022
Nat Methods. 2008 Feb;5(2):155-7
pubmed: 18193054
Traffic. 2015 Jan;16(1):85-98
pubmed: 25369882
Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19848-53
pubmed: 19903887
Phys Rev Lett. 2005 May 6;94(17):178104
pubmed: 15904340
Nat Protoc. 2019 Apr;14(4):1054-1083
pubmed: 30842616
Mol Biol Cell. 2017 Jun 1;28(11):1507-1518
pubmed: 28404749
Anal Chem. 1998 Jul 1;70(13):2651-9
pubmed: 21644785
J Mol Biol. 2008 Sep 12;381(4):867-80
pubmed: 18598704
J Am Chem Soc. 2010 Mar 24;132(11):3973-9
pubmed: 20178365
Biophys J. 1997 Apr;72(4):1878-86
pubmed: 9083691
Biophys J. 2004 Nov;87(5):3518-24
pubmed: 15339818
J Biol Chem. 2011 Nov 25;286(47):40509-19
pubmed: 21976670
PLoS One. 2015 Oct 20;10(10):e0140685
pubmed: 26484888
J Mol Biol. 2000 May 12;298(4):677-89
pubmed: 10788329
J Biol Chem. 2005 Jul 1;280(26):24404-11
pubmed: 15866874
Nature. 2009 Feb 26;457(7233):1159-62
pubmed: 19098897