Effect of fixed orthodontic appliances on gingival status and oral microbiota: a pilot study.
Candida sp.
Oral microbiome
Oral microbiota
Orthodontic treatment
Plaque index
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
27 10 2022
27 10 2022
Historique:
received:
09
06
2022
accepted:
18
10
2022
entrez:
28
10
2022
pubmed:
29
10
2022
medline:
1
11
2022
Statut:
epublish
Résumé
This pilot study aimed to investigate how fixed orthodontic appliances simultaneously applied on the upper and lower arches affect the oral environment in the medium term. The oral status of 30 orthodontic patients was evaluated using the number of decay-missing-filled teeth (DMFT), plaque (PI), and gingival indices (GI) before bonding of fixed orthodontic appliances (T0) and during the therapy (T1). Besides, the gingival crevicular fluid (GCF) and a dental plaque were collected. Samples were analyzed for selected Candida sp. and for 10 selected oral bacteria using mass spectroscopy and multiplex polymerase chain reaction, respectively. In 60% of patients, deterioration of the oral status (demonstrated by the increase in PI) was recorded (p < 0.05). Moreover, the changes in PI correlated with those of GI (p < 0.001). At the T1 time point, the mean representation of Actinomyces sp. in the total prokaryotic DNA in GCF and dental plaque of individual patients increased compared to T0 (p < 0.05). The probability of finding any of the 7 selected periodontal bacteria combined with Candida sp. was 10 times higher in patients in whom PI deteriorated between T0 and T1 (p < 0.01). Changes in the oral microbial diversity and an increase in PI were observed in the medium term after bonding of orthodontic appliance. Our study highlights the importance of a complex approach in this type of research as the association between clinical characteristics and combined microbial parameters is higher than when evaluated separately.
Sections du résumé
BACKGROUND
This pilot study aimed to investigate how fixed orthodontic appliances simultaneously applied on the upper and lower arches affect the oral environment in the medium term.
METHODS
The oral status of 30 orthodontic patients was evaluated using the number of decay-missing-filled teeth (DMFT), plaque (PI), and gingival indices (GI) before bonding of fixed orthodontic appliances (T0) and during the therapy (T1). Besides, the gingival crevicular fluid (GCF) and a dental plaque were collected. Samples were analyzed for selected Candida sp. and for 10 selected oral bacteria using mass spectroscopy and multiplex polymerase chain reaction, respectively.
RESULTS
In 60% of patients, deterioration of the oral status (demonstrated by the increase in PI) was recorded (p < 0.05). Moreover, the changes in PI correlated with those of GI (p < 0.001). At the T1 time point, the mean representation of Actinomyces sp. in the total prokaryotic DNA in GCF and dental plaque of individual patients increased compared to T0 (p < 0.05). The probability of finding any of the 7 selected periodontal bacteria combined with Candida sp. was 10 times higher in patients in whom PI deteriorated between T0 and T1 (p < 0.01).
CONCLUSIONS
Changes in the oral microbial diversity and an increase in PI were observed in the medium term after bonding of orthodontic appliance. Our study highlights the importance of a complex approach in this type of research as the association between clinical characteristics and combined microbial parameters is higher than when evaluated separately.
Identifiants
pubmed: 36303145
doi: 10.1186/s12903-022-02511-9
pii: 10.1186/s12903-022-02511-9
pmc: PMC9615380
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
455Informations de copyright
© 2022. The Author(s).
Références
Mycoses. 2019 Mar;62(3):247-251
pubmed: 30561858
J Periodontol. 1967 Nov-Dec;38(6):Suppl:610-6
pubmed: 5237684
Int J Dent Hyg. 2008 May;6(2):129-36
pubmed: 18412726
Acta Odontol Scand. 2015;73(7):496-502
pubmed: 25631494
Am J Orthod Dentofacial Orthop. 1999 Apr;115(4):423-8
pubmed: 10194288
Acta Microbiol Immunol Hung. 2016 Mar;63(1):103-13
pubmed: 27020873
PLoS One. 2013;8(3):e58487
pubmed: 23520516
J Bacteriol. 2010 Oct;192(19):5002-17
pubmed: 20656903
J Oral Microbiol. 2018 Jul 03;10(1):1476645
pubmed: 29988826
Angle Orthod. 2019 Jan;89(1):25-32
pubmed: 30239218
J Indian Soc Pedod Prev Dent. 2017 Jan-Mar;35(1):34-40
pubmed: 28139480
J Clin Periodontol. 1997 Sep;24(9 Pt 1):610-7
pubmed: 9378831
BMC Oral Health. 2020 Oct 10;20(1):277
pubmed: 33036600
Am J Orthod Dentofacial Orthop. 2004 Sep;126(3):363-6
pubmed: 15356501
J Clin Periodontol. 1983 Jul;10(4):380-8
pubmed: 6577031
Angle Orthod. 2009 Nov;79(6):1149-55
pubmed: 19852608
J Clin Pediatr Dent. 2021 Apr 1;45(2):135-139
pubmed: 33951170
J Clin Exp Dent. 2013 Dec 01;5(5):e279-86
pubmed: 24455095
Am J Orthod Dentofacial Orthop. 2009 Jan;135(1):95-8
pubmed: 19121507
BMC Oral Health. 2016 Oct 28;16(1):112
pubmed: 27793138
Am J Orthod Dentofacial Orthop. 2006 Sep;130(3):275.e17-22
pubmed: 16979483
Head Face Med. 2016 Oct 28;12(1):28
pubmed: 27793169
Oral Health Prev Dent. 2021;19(1):67
pubmed: 33491380
Rev Chilena Infectol. 2016 Jun;33(3):293-7
pubmed: 27598278
J Periodontol (1930). 1965 May-Jun;36:177-87
pubmed: 14296927
Public Health Nutr. 2001 Apr;4(2B):569-91
pubmed: 11683551
Angle Orthod. 2012 Mar;82(2):254-60
pubmed: 21827233
J Clin Med. 2021 Feb 16;10(4):
pubmed: 33669186
Diagnostics (Basel). 2019 Dec 22;10(1):
pubmed: 31877891
J Dent Sci. 2016 Mar;11(1):17-22
pubmed: 30894940
J Dent (Tehran). 2016 Jan;13(1):18-22
pubmed: 27536324
Srp Arh Celok Lek. 2014 May-Jun;142(5-6):301-5
pubmed: 25033585
BMC Oral Health. 2017 Jun 2;17(1):90
pubmed: 28576147
Dent Res J (Isfahan). 2016 Jul-Aug;13(4):309-14
pubmed: 27605987
J Appl Oral Sci. 2014 Jan-Feb;22(1):38-43
pubmed: 24626247
Aust Orthod J. 1999 Apr;15(4):229-34
pubmed: 11933357
J Clin Exp Dent. 2020 Nov 01;12(11):e1071-e1077
pubmed: 33262874
Angle Orthod. 2011 Jul;81(4):609-15
pubmed: 21306224
Ann Agric Environ Med. 2019 Jun 17;26(2):231-235
pubmed: 31232051