Reversible and Irreversible Effects of Electroporation on Contractility and Calcium Homeostasis in Isolated Cardiac Ventricular Myocytes.

Rats Animals Myocytes, Cardiac / metabolism Calcium / metabolism Heart Ventricles / metabolism Electroporation Homeostasis
calcium electroporation homeostasis myocytes, cardiac sacromere

Journal

Circulation. Arrhythmia and electrophysiology
ISSN: 1941-3084
Titre abrégé: Circ Arrhythm Electrophysiol
Pays: United States
ID NLM: 101474365

Informations de publication

Date de publication:
11 2022
Historique:
pubmed: 29 10 2022
medline: 19 11 2022
entrez: 28 10 2022
Statut: ppublish

Résumé

Irreversible electroporation is an energy form utilizing high-voltage pulsed electric field, leading to cellular homeostasis disruption and cell death. Recently, irreversible electroporation has shown promising results for the treatment of cardiac arrhythmias. However, reversible and irreversible effects of pulsed electric field on cardiac myocytes remain poorly understood. Here, we evaluated the influence of a monophasic single electric pulse (EP) on the contractility, Ca Isolated rat left ventricular myocytes were electroporated using single monophasic EP of different durations and voltages. Sarcomere length and intracellular Ca Electroporation led to an immediate increase in intracellular Ca Sublethal EP affected rat left ventricular myocytes contractility and disrupted Ca

Sections du résumé

BACKGROUND
Irreversible electroporation is an energy form utilizing high-voltage pulsed electric field, leading to cellular homeostasis disruption and cell death. Recently, irreversible electroporation has shown promising results for the treatment of cardiac arrhythmias. However, reversible and irreversible effects of pulsed electric field on cardiac myocytes remain poorly understood. Here, we evaluated the influence of a monophasic single electric pulse (EP) on the contractility, Ca
METHODS
Isolated rat left ventricular myocytes were electroporated using single monophasic EP of different durations and voltages. Sarcomere length and intracellular Ca
RESULTS
Electroporation led to an immediate increase in intracellular Ca
CONCLUSIONS
Sublethal EP affected rat left ventricular myocytes contractility and disrupted Ca

Identifiants

DOI: 10.1161/CIRCEP.122.011131 PMID: 36306333 PMC: PMC9665944
pubmed: 36306333
doi: 10.1161/CIRCEP.122.011131
pmc: PMC9665944
doi:

Substances chimiques

Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e011131

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Auteurs

Sébastien Chaigne (S)

IHU LIRYC Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Université, Bordeaux, France (S.C., M.H., O.B., D.B.).
University of Bordeaux, Inserm, CRCTB U1045, Bordeaux, France (S.C., M.H., O.B., D.B.).
CHU de Bordeaux, Hôpital Cardiologique Haut-Lévêque, Pessac, France (S.C., M.H.).
ORCID: 0000-0002-7141-8469

Daniel C Sigg (DC)

Medtronic Cardiac Ablation Solutions, Minneapolis, MN (D.C.S., M.T.S.).
ORCID: 0000-0002-9556-0849

Mark T Stewart (MT)

Medtronic Cardiac Ablation Solutions, Minneapolis, MN (D.C.S., M.T.S.).
ORCID: 0000-0003-1717-195X

Mélèze Hocini (M)

IHU LIRYC Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Université, Bordeaux, France (S.C., M.H., O.B., D.B.).
University of Bordeaux, Inserm, CRCTB U1045, Bordeaux, France (S.C., M.H., O.B., D.B.).
CHU de Bordeaux, Hôpital Cardiologique Haut-Lévêque, Pessac, France (S.C., M.H.).
ORCID: 0000-0002-7957-8766

Tina Batista Napotnik (T)

Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia (T.B.N., D.M.).

Damijan Miklavčič (D)

Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia (T.B.N., D.M.).
ORCID: 0000-0003-3506-9449

Olivier Bernus (O)

IHU LIRYC Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Université, Bordeaux, France (S.C., M.H., O.B., D.B.).
University of Bordeaux, Inserm, CRCTB U1045, Bordeaux, France (S.C., M.H., O.B., D.B.).
ORCID: 0000-0003-3917-5791

David Benoist (D)

IHU LIRYC Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Université, Bordeaux, France (S.C., M.H., O.B., D.B.).
University of Bordeaux, Inserm, CRCTB U1045, Bordeaux, France (S.C., M.H., O.B., D.B.).
ORCID: 0000-0002-7159-3451

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Reversible and Irreversible Effects of...
questionsmedicales.fr › Eucaryotes › Animaux › Reversible and Irreversible Effects of...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Reversible and Irreversible Effects of...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Reversible and Irreversible Effects of...
questionsmedicales.fr › Système cardiovasculaire › Coeur › Ventricules cardiaques › Reversible and Irreversible Effects of...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électroporation › Reversible and Irreversible Effects of...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Reversible and Irreversible Effects of...