A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 10 2022
28 10 2022
Historique:
received:
06
04
2022
accepted:
06
10
2022
entrez:
28
10
2022
pubmed:
29
10
2022
medline:
2
11
2022
Statut:
epublish
Résumé
The experimental need to engineer the genome both in time and space, has led to the development of several photoactivatable Cre recombinase systems. However, the combination of inefficient and non-intentional background recombination has prevented thus far the wide application of these systems in biological and biomedical research. Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre). Following extensive characterization in cancer cell and organoid systems, we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis in vivo and positional cell-tracing by intravital microscopy. These experiments illustrate how newly formed HrasV12 mutant cells follow an unnatural movement towards the interfollicular dermis. Together, we develop an efficient photoactivatable Cre recombinase mouse model and illustrate how this model is a powerful genome-editing tool for biological and biomedical research.
Identifiants
pubmed: 36307419
doi: 10.1038/s41467-022-33863-z
pii: 10.1038/s41467-022-33863-z
pmc: PMC9616875
doi:
Substances chimiques
Cre recombinase
EC 2.7.7.-
Doxycycline
N12000U13O
Integrases
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6442Informations de copyright
© 2022. The Author(s).
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