Biophysical and biochemical insights in the design of immunoassays.
Infectious diseases
Lateral flow immunoassay
Nanoparticle-antibody conjugates
Paperfluidic
Protein-ligand affinity
Rapid diagnostic
Viral biomarkers
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
11
08
2022
revised:
17
10
2022
accepted:
21
10
2022
pubmed:
30
10
2022
medline:
18
11
2022
entrez:
29
10
2022
Statut:
ppublish
Résumé
Rapid antigen assays have been attractive for decentralized, point of care diagnostics because of their low cost, robustness, and ease of use. The development of a diagnostic assay for a newly emerging infectious disease needs to take into account the progression of a disease, whether there is human to human transmission, and patient biomarker levels with time, and these all impact the choice of antigen targets and affinity agents. The factors involved in the biophysical design of rapid antigen immunoassays are discussed, focusing on antigen selection and designing for cross-reactivity. State of the art in the biophysical characterization of protein-ligand or antigen-antibody interactions, the different types of affinity agents used in immunoassays, and biochemical conjugation strategies are described. Antigen choice is a critical factor in immunoassay diagnostic development, and should account for the properties of the virion, virus, and disease progression. Biophysical and biochemical aspects of immunoassays are critical for performance. This review can serve as an instructive guide to aid in diagnostic development for future emerging diseases.
Sections du résumé
BACKGROUND
Rapid antigen assays have been attractive for decentralized, point of care diagnostics because of their low cost, robustness, and ease of use. The development of a diagnostic assay for a newly emerging infectious disease needs to take into account the progression of a disease, whether there is human to human transmission, and patient biomarker levels with time, and these all impact the choice of antigen targets and affinity agents.
SCOPE OF REVIEW
The factors involved in the biophysical design of rapid antigen immunoassays are discussed, focusing on antigen selection and designing for cross-reactivity. State of the art in the biophysical characterization of protein-ligand or antigen-antibody interactions, the different types of affinity agents used in immunoassays, and biochemical conjugation strategies are described.
MAJOR CONCLUSIONS
Antigen choice is a critical factor in immunoassay diagnostic development, and should account for the properties of the virion, virus, and disease progression. Biophysical and biochemical aspects of immunoassays are critical for performance.
GENERAL SIGNIFICANCE
This review can serve as an instructive guide to aid in diagnostic development for future emerging diseases.
Identifiants
pubmed: 36309294
pii: S0304-4165(22)00184-2
doi: 10.1016/j.bbagen.2022.130266
pii:
doi:
Substances chimiques
Proteins
0
Biomarkers
0
Types de publication
Journal Article
Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
130266Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.