Music improves the therapeutic effects of bevacizumab in rats with glioblastoma: Modulation of drug distribution to the brain.
bevacizumab
blood-brain barrier
brain drug delivery
music
rat glioblastoma
Journal
Frontiers in oncology
ISSN: 2234-943X
Titre abrégé: Front Oncol
Pays: Switzerland
ID NLM: 101568867
Informations de publication
Date de publication:
2022
2022
Historique:
received:
02
08
2022
accepted:
29
08
2022
entrez:
31
10
2022
pubmed:
1
11
2022
medline:
1
11
2022
Statut:
epublish
Résumé
The development of new methods for modulation of drug distribution across to the brain is a crucial step in the effective therapies for glioblastoma (GBM). In our previous work, we discovered the phenomenon of music-induced opening of the blood-brain barrier (OBBB) in healthy rodents. In this pilot study on rats, we clearly demonstrate that music-induced BBB opening improves the therapeutic effects of bevacizumab (BZM) in rats with GBM The experiments were performed on Wistar male rats (200-250 g, n=161) using transfected C6-TagRFP cell line and the loud rock music for OBBB. The OBBB was assessed by spectrofluorometric assay of Evans Blue (EB) extravasation and confocal imaging of fluorescent BZM (fBZM) delivery into the brain. Additionally, distribution of fBZM and Omniscan in the brain was studied using fluorescent and magnetic resonance imaging (MRI), respectively. To analyze the therapeutic effects of BZM on the GBM growth in rats without and with OBBB, the GBM volume (MRI scans), as well as immunohistochemistry assay of proliferation (Ki67 marker) and apoptosis (Bax marker) in the GBM cells were studied. The Mann-Whitney-Wilcoxon test was used for all analysis, the significance level was p < 0.05, n=7 in each group. Our finding clearly demonstrates that music-induced OBBB increases the delivery of EB into the brain tissues and the extravasation of BZM into the brain around the cerebral vessels of rats with GBM. Music significantly increases distribution of tracers (fBZM and Omniscan) in the rat brain through the pathways of brain drainage system (perivascular and lymphatic), which are an important route of drug delivery into the brain. The music-induced OBBB improves the suppressive effects of BZM on the GBM volume and the cellular mechanisms of tumor progression that was accompanied by higher survival among rats in the GBM+BZM+Music group We hypothesized that music improves the therapeutic effects of BZM
Sections du résumé
Background
UNASSIGNED
The development of new methods for modulation of drug distribution across to the brain is a crucial step in the effective therapies for glioblastoma (GBM). In our previous work, we discovered the phenomenon of music-induced opening of the blood-brain barrier (OBBB) in healthy rodents. In this pilot study on rats, we clearly demonstrate that music-induced BBB opening improves the therapeutic effects of bevacizumab (BZM) in rats with GBM
Methods
UNASSIGNED
The experiments were performed on Wistar male rats (200-250 g, n=161) using transfected C6-TagRFP cell line and the loud rock music for OBBB. The OBBB was assessed by spectrofluorometric assay of Evans Blue (EB) extravasation and confocal imaging of fluorescent BZM (fBZM) delivery into the brain. Additionally, distribution of fBZM and Omniscan in the brain was studied using fluorescent and magnetic resonance imaging (MRI), respectively. To analyze the therapeutic effects of BZM on the GBM growth in rats without and with OBBB, the GBM volume (MRI scans), as well as immunohistochemistry assay of proliferation (Ki67 marker) and apoptosis (Bax marker) in the GBM cells were studied. The Mann-Whitney-Wilcoxon test was used for all analysis, the significance level was p < 0.05, n=7 in each group.
Results
UNASSIGNED
Our finding clearly demonstrates that music-induced OBBB increases the delivery of EB into the brain tissues and the extravasation of BZM into the brain around the cerebral vessels of rats with GBM. Music significantly increases distribution of tracers (fBZM and Omniscan) in the rat brain through the pathways of brain drainage system (perivascular and lymphatic), which are an important route of drug delivery into the brain. The music-induced OBBB improves the suppressive effects of BZM on the GBM volume and the cellular mechanisms of tumor progression that was accompanied by higher survival among rats in the GBM+BZM+Music group
Conclusion
UNASSIGNED
We hypothesized that music improves the therapeutic effects of BZM
Identifiants
pubmed: 36313687
doi: 10.3389/fonc.2022.1010188
pmc: PMC9606698
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1010188Informations de copyright
Copyright © 2022 Semyachkina-Glushkovskaya, Diduk, Anna, Elina, Artem, Khorovodov, Shirokov, Fedosov, Dubrovsky, Blokhina, Terskov, Navolokin, Evsukova, Elovenko, Adushkina and Kurths.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Clin Oncol. 2009 Oct 1;27(28):4733-40
pubmed: 19720927
Noise Health. 2004 Apr-Jun;6(23):59-72
pubmed: 15273024
Naunyn Schmiedebergs Arch Pharmacol. 1991 Jun;343(6):633-7
pubmed: 1658663
PLoS One. 2012;7(5):e36555
pubmed: 22590566
Nature. 2019 Aug;572(7767):62-66
pubmed: 31341278
Neuro Oncol. 2018 Jan 22;20(2):184-191
pubmed: 29016900
Proc Biol Sci. 2020 Dec 23;287(1941):20202337
pubmed: 33323086
Nat Med. 2021 Mar;27(3):411-418
pubmed: 33462448
Brain Res. 1994 May 16;646(1):95-9
pubmed: 8055344
Circulation. 2013 Apr 23;127(16):1712-22
pubmed: 23529610
Int J Clin Exp Pathol. 2015 May 01;8(5):5291-9
pubmed: 26191230
Comp Med. 2005 Feb;55(1):12-23
pubmed: 15766204
N Engl J Med. 2011 Jul 14;365(2):e2
pubmed: 21707384
J Cereb Blood Flow Metab. 2017 Mar;37(3):762-775
pubmed: 28155574
Int J Nanomedicine. 2014 Sep 18;9:4437-48
pubmed: 25258533
Prog Neurobiol. 2009 Aug;88(4):272-85
pubmed: 19486920
Cancer Drug Resist. 2021;4:17-43
pubmed: 34337348
Brain. 2006 Oct;129(Pt 10):2533-53
pubmed: 16845129
Invest Ophthalmol Vis Sci. 2009 Jul;50(7):3438-46
pubmed: 19255151
J Clin Oncol. 2013 Jun 10;31(17):2205-18
pubmed: 23669226
Physiol Rev. 2011 Jul;91(3):1071-121
pubmed: 21742796
J Ophthalmol. 2015;2015:934963
pubmed: 26491557
Noise Health. 2011 Sep-Oct;13(54):333-9
pubmed: 21959113
Expert Opin Drug Deliv. 2016 Jul;13(7):963-75
pubmed: 27020469
Neoplasia. 2000 Jul-Aug;2(4):306-14
pubmed: 11005565
Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12873-8
pubmed: 17646643
AJNR Am J Neuroradiol. 2016 Sep;37(9):1650-6
pubmed: 27173366
Acta Psychol (Amst). 2001 Apr;107(1-3):293-321
pubmed: 11388140
Nat Commun. 2019 Sep 26;10(1):4373
pubmed: 31558719
Korean J Ophthalmol. 2013 Dec;27(6):425-32
pubmed: 24311928
Science. 2005 Jan 7;307(5706):58-62
pubmed: 15637262
Front Cell Neurosci. 2014 Dec 12;8:418
pubmed: 25565956
Drug Deliv. 2019 Dec;26(1):551-565
pubmed: 31928355
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Clin Cancer Res. 2020 Jan 1;26(1):206-212
pubmed: 31558474
Nature. 2011 May 19;473(7347):298-307
pubmed: 21593862
Brain Tumor Res Treat. 2017 Apr;5(1):1-9
pubmed: 28516072
Aging Dis. 2020 Feb 1;11(1):200-211
pubmed: 32010493
Neuroreport. 2012 Aug 22;23(12):699-701
pubmed: 22729097
Cancer Res. 2005 Feb 1;65(3):671-80
pubmed: 15705858
Sci Rep. 2019 Oct 15;9(1):14815
pubmed: 31616011
N Engl J Med. 2017 Nov 16;377(20):1954-1963
pubmed: 29141164
Nat Rev Cancer. 2020 Jan;20(1):26-41
pubmed: 31601988
J Neurosci Methods. 1983 May;8(1):41-9
pubmed: 6876872
N Engl J Med. 2011 Oct 13;365(15):1454-5
pubmed: 21995408
Sci Rep. 2014 Oct 10;4:6588
pubmed: 25300427
Mol Med Rep. 2012 Jan;5(1):73-7
pubmed: 21946944
Proc Natl Acad Sci U S A. 2017 Jan 3;114(1):E75-E84
pubmed: 27994152
J Clin Oncol. 2009 Feb 10;27(5):740-5
pubmed: 19114704
N Engl J Med. 2014 Feb 20;370(8):699-708
pubmed: 24552317
J Am Assoc Lab Anim Sci. 2010 Sep;49(5):592-7
pubmed: 20858361
Nat Commun. 2018 Jul 25;9(1):2336
pubmed: 30046032
Expert Rev Mol Med. 2011 May 13;13:e17
pubmed: 21676290
Nat Rev Neurosci. 2007 Aug;8(8):610-22
pubmed: 17643088
Nature. 2018 Aug;560(7717):185-191
pubmed: 30046111
Cancers (Basel). 2018 Oct 11;10(10):
pubmed: 30314362
Oncologist. 2000;5(2):144-51
pubmed: 10794805
J Acoust Soc Am. 2019 Nov;146(5):3692
pubmed: 31795685
Behav Brain Res. 1996 Apr;76(1-2):191-7
pubmed: 8734053
Adv Exp Med Biol. 2021;1269:197-202
pubmed: 33966217
J Cereb Blood Flow Metab. 1985 Sep;5(3):401-12
pubmed: 3928638
Trends Cogn Sci. 2006 Jan;10(1):14-23
pubmed: 16321563
Acta Neurol Scand. 1980 Aug;62(2):96-102
pubmed: 6111172
Toxicol Appl Pharmacol. 2000 May 15;165(1):99-105
pubmed: 10814558
Front Neurosci. 2015 Oct 29;9:385
pubmed: 26578854
N Engl J Med. 2014 Feb 20;370(8):709-22
pubmed: 24552318