Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation.
adeno-associated virus
albumin
cerebral blood flow
chronic
hepatocytes
hyperemia
in vivo imaging
microcirculation
recombinant protein
vasculature
Journal
Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676
Informations de publication
Date de publication:
24 10 2022
24 10 2022
Historique:
received:
07
04
2022
revised:
11
08
2022
accepted:
01
09
2022
entrez:
31
10
2022
pubmed:
1
11
2022
medline:
1
11
2022
Statut:
epublish
Résumé
Studying blood microcirculation is vital for gaining insights into vascular diseases. Blood flow imaging in deep tissue is currently achieved by acute administration of fluorescent dyes in the blood plasma. This is an invasive process, and the plasma fluorescence decreases within an hour of administration. Here, we report an approach for the longitudinal study of vasculature. Using a single intraperitoneal or intravenous administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to chronically label the blood circulation in mice. This approach allows for longitudinal observation of circulation from 2 weeks to over 4 months after vector administration. We demonstrate the chronic assessment of vascular functions including functional hyperemia and vascular plasticity in micro- and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease animal models.
Identifiants
pubmed: 36313804
doi: 10.1016/j.crmeth.2022.100302
pii: S2667-2375(22)00184-9
pmc: PMC9606131
doi:
Banques de données
figshare
['10.6084/m9.figshare.20629626']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Pagination
100302Subventions
Organisme : NINDS NIH HHS
ID : U19 NS128613
Pays : United States
Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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