Characterization of B-cell and T-cell responses to a tetravalent dengue purified inactivated vaccine in healthy adults.


Journal

NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863

Informations de publication

Date de publication:
31 Oct 2022
Historique:
received: 05 08 2021
accepted: 08 09 2022
entrez: 1 11 2022
pubmed: 2 11 2022
medline: 2 11 2022
Statut: epublish

Résumé

The increasing global impact of dengue underscores the need for a dengue virus (DENV) vaccine. We assessed B-cell and T-cell responses following vaccination with four formulations of a tetravalent dengue purified inactivated vaccine (DPIV) in dengue-primed and dengue-naive adults from two studies (NCT01666652, NCT01702857). Frequencies of DPIV-induced memory B cells specific to each DENV serotype remained high up to 12 months post-vaccination, and were higher in the dengue-primed than dengue-naive adults. A subsequent DPIV booster dose induced strong anamnestic B-cell responses. Multifunctional CD4+ T cells (predominantly expressing IL-2) were induced by DPIV, with higher frequencies in dengue-primed adults. DPIV-induced CD4+ T cells also demonstrated in vitro proliferative capacity and antigen-specific production of GM-CSF, IFN-γ, and IL-13. CD8+ T-cell responses were undetectable in dengue-naive adults and low in dengue-primed individuals. B- and T-cell responses persisted up to 12 months post-vaccination in both dengue-primed and dengue-naive adults.

Identifiants

pubmed: 36316335
doi: 10.1038/s41541-022-00537-2
pii: 10.1038/s41541-022-00537-2
pmc: PMC9622737
doi:

Types de publication

Journal Article

Langues

eng

Pagination

132

Informations de copyright

© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022.

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Auteurs

Heather Friberg (H)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. heather.l.friberg-robertson.civ@health.mil.

Morgan Gargulak (M)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Amanda Kong (A)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Leyi Lin (L)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Luis J Martinez (LJ)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Alexander C Schmidt (AC)

GSK, Rockville, MD, USA.

Robert M Paris (RM)

GSK, Rockville, MD, USA.

Richard G Jarman (RG)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Clemente Diaz (C)

University of Puerto Rico School of Medicine, San Juan, Puerto Rico.

Stephen J Thomas (SJ)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Philippe Moris (P)

GSK, Rixensart, Belgium.

Jeffrey R Currier (JR)

Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Classifications MeSH