A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells.

Humans Pluripotent Stem Cells Mesenchymal Stem Cells Cell Differentiation Chondrogenesis Limb Buds Homeodomain Proteins
Cell culture Cell differentiation Developmental biology Organoids Stem cells Tissue engineering

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 12 2022
Historique:
entrez: 1 11 2022
pubmed: 2 11 2022
medline: 3 11 2022
Statut: epublish

Résumé

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1

Identifiants

DOI: 10.1016/j.xpro.2022.101786 PMID: 36317170 PMC: PMC9617203
pubmed: 36317170
doi: 10.1016/j.xpro.2022.101786
pii: S2666-1667(22)00666-9
pmc: PMC9617203
pii:
doi:

Substances chimiques

PRRX1 protein, human 0
Homeodomain Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101786

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

T. Takao, D.Y., and T. Takarada have a patent pending related to this work (PCT/JP2020/03551).

Références

Cell. 2016 Jul 14;166(2):451-467
pubmed: 27419872
Nat Biomed Eng. 2021 Aug;5(8):926-940
pubmed: 34373601
Int J Mol Sci. 2022 Feb 28;23(5):
pubmed: 35269809

Auteurs

Tomoka Takao (T)

Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: ttakao@okayama-u.ac.jp.

Daisuke Yamada (D)

Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: dyamada@okayama-u.ac.jp.

Takeshi Takarada (T)

Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: takarada@okayama-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système locomoteur › Développement locomoteur › Chondrogenèse › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Structures de l'embryon › Bourgeons de membre › A protocol to induce expandable limb-bud...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Protéines à homéodomaine › A protocol to induce expandable limb-bud...