NEDD1-S411 phosphorylation plays a critical function in the coordination of microtubule nucleation during mitosis.
Centrosome
Microtubule branching
Microtubule nucleation
NEDD1 phosphorylation
RanGTP
Spindle
Journal
Biology open
ISSN: 2046-6390
Titre abrégé: Biol Open
Pays: England
ID NLM: 101578018
Informations de publication
Date de publication:
01 11 2022
01 11 2022
Historique:
received:
07
06
2022
accepted:
11
10
2022
pubmed:
2
11
2022
medline:
22
11
2022
entrez:
1
11
2022
Statut:
ppublish
Résumé
During mitosis, spindle assembly relies on centrosomal and acentrosomal microtubule nucleation pathways that all require the γ-Tubulin Ring Complex (γ-TuRC) and its adaptor protein NEDD1. The activity of these different pathways needs to be coordinated to ensure bipolar spindle assembly ( Cavazza et al., 2016) but the underlying mechanism is still unclear. Previous studies have identified three sites in NEDD1 (S377, S405 and S411) that when phosphorylated drive MT nucleation at the centrosomes, around the chromosomes and on pre-existing MTs respectively ( Lüders et al., 2006; Pinyol et al., 2013; Sdelci et al., 2012). Here we aimed at getting additional insights into the mechanism that coordinates the different MT nucleation pathways in dividing cells using a collection of HeLa stable inducible cell lines expressing NEDD1 phospho-variants at these three sites and Xenopus egg extracts. Our results provide further support for the essential role of phosphorylation at the three residues. Moreover, we directly demonstrate that S411 phosphorylation is essential for MT branching using TIRF microscopy in Xenopus egg extracts and we show that it plays a crucial role in ensuring the balance between centrosome and chromosome-dependent MT nucleation required for bipolar spindle assembly in mitotic cells.
Identifiants
pubmed: 36318115
pii: 278477
doi: 10.1242/bio.059474
pmc: PMC9836086
pii:
doi:
Substances chimiques
Microtubule-Associated Proteins
0
NEDD1 protein, human
0
Tubulin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Economy and competitiveness
ID : BFU2015-68726-P
Organisme : Ministry of Science, Innovation and Universities
ID : PGC2018-096976-B-I00
Organisme : Generalitat de Catalunya
ID : AGAUR-SGR 478
Organisme : Spanish Ministry of Economy, Industry and Competitiveness (MEIC)
Organisme : Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa'
Organisme : CERCA Programme / Generalitat de Catalunya
Organisme : Spanish Ministry of Economy and competitiveness (MINECO)
ID : BFU2015-68726-P
Organisme : Generalitat de Catalunya and intramural funds from the Centre for Genomic Regulation
Organisme : CRG: Centre de Regulacio Genomica
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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