Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 11 2022
01 11 2022
Historique:
received:
15
06
2022
accepted:
20
10
2022
entrez:
2
11
2022
pubmed:
3
11
2022
medline:
4
11
2022
Statut:
epublish
Résumé
Mouse cloning by nuclear transfer using freeze-drying (FD) somatic cells is now possible, but the success rate is significantly lower than that of FD spermatozoa. Because spermatozoa, unlike somatic cells, are haploid cells with hardened nuclei due to protamine, the factors responsible for their tolerance to FD treatment remain unclear. In this study, we attempt to produce offspring from FD spermatid, a haploid sperm progenitor cell whose nuclei, like somatic cells, have not yet been replaced by protamine. We developed a method for collecting FD spermatids from testicular suspension. Despite the significantly lower success rate than that of FD spermatozoa, healthy offspring were obtained when FD spermatids were injected into oocytes. Offspring were also obtained from FD spermatids derived from immature male mice that had not yet produced spermatozoa. These results suggest that nuclear protaminization, rather than haploid nuclei, is one of the key processes responsible for tolerance to FD treatment.
Identifiants
pubmed: 36319672
doi: 10.1038/s41598-022-22850-5
pii: 10.1038/s41598-022-22850-5
pmc: PMC9626645
doi:
Substances chimiques
Protamines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18430Subventions
Organisme : Takahashi Industrial and Economic Research Foundation
ID : 189
Organisme : Japan Society for the Promotion of Science
ID : 15KT0086
Organisme : Japan Society for the Promotion of Science
ID : 17K08134
Organisme : Canon Foundation
ID : M20-0008
Informations de copyright
© 2022. The Author(s).
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