Full regeneration of descending corticotropin-releasing hormone axons after a complete spinal cord injury in lampreys.
Axon regeneration
CRH
Descending axons
Immunohistochemistry
Lamprey
Spinal cord injury
Journal
Computational and structural biotechnology journal
ISSN: 2001-0370
Titre abrégé: Comput Struct Biotechnol J
Pays: Netherlands
ID NLM: 101585369
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
09
2022
revised:
14
10
2022
accepted:
14
10
2022
entrez:
2
11
2022
pubmed:
3
11
2022
medline:
3
11
2022
Statut:
epublish
Résumé
Sea lampreys are a vertebrate model of interest for the study of spontaneous axon regeneration after spinal cord injury (SCI). Axon regeneration research in lampreys has focused on the study of giant descending neurons, but less so on neurochemically-distinct descending neuronal populations with small caliber axons. Corticotropin-releasing hormone (CRH) is a neuropeptide that regulates the stress response or locomotion. CRH is also a neuropeptide of interest in the SCI context because descending CRHergic projections from the Barrington's nucleus control micturition behavior in mammals. Recent work from our group revealed that in sea lampreys the CRHergic innervation of the spinal cord is only of descending origin. Thus, the lack of intrinsic CRH spinal cord neurons provides the opportunity to analyze the regeneration of this descending system by using immunofluorescence methods. Here, we used an antibody against the sea lamprey mature CRH peptide, confocal microscopy, lightning adaptive deconvolution, and ImageJ to analyze the regenerative capacity of the descending CRH-immunoreactive (-ir) axons of larval sea lampreys after a complete SCI at the level of the fifth gill. At 10 weeks post-lesion, when behavioral analyses showed that injured animals had recovered normal appearing locomotion, our results revealed a full recovery of the number of CRH-ir profiles (axons) at the level of the sixth gill. Thus, the CRH descending axons of lampreys fully regenerate after a complete SCI. Our study provides a new model to study spontaneous and successful axonal regeneration in a specific neuronal type with small caliber axons by using simple immunohistochemical methods.
Identifiants
pubmed: 36320936
doi: 10.1016/j.csbj.2022.10.020
pii: S2001-0370(22)00467-6
pmc: PMC9596600
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5690-5697Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Neural Plast. 2015;2015:350750
pubmed: 25861481
Prog Brain Res. 1994;103:203-17
pubmed: 7886205
Spinal Cord. 2010 Dec;48(12):850-6
pubmed: 20458328
Neuroscience. 2013 Aug 15;245:74-89
pubmed: 23603516
Neuroscience. 2019 Jun 1;408:430-447
pubmed: 30943435
Exp Neurol. 2011 Apr;228(2):283-93
pubmed: 21316361
J Neurotrauma. 2005 Jan;22(1):172-88
pubmed: 15665611
Restor Neurol Neurosci. 2006;24(2):69-78
pubmed: 16720943
J Comp Neurol. 2012 Jun 1;520(8):1751-71
pubmed: 22120153
J Neurotrauma. 2020 Mar 15;37(6):899-903
pubmed: 31469029
PLoS One. 2019 Jan 30;14(1):e0204193
pubmed: 30699109
J Vis Exp. 2014 Oct 14;(92):e51494
pubmed: 25350040
J Neurosci. 1997 Jul 1;17(13):5206-20
pubmed: 9185558
Glia. 2014 Aug;62(8):1254-69
pubmed: 24733772
Neuroscience. 2011 Dec 29;199:563-76
pubmed: 21952133
Front Cell Dev Biol. 2020 Mar 20;8:173
pubmed: 32266257
Cell Death Dis. 2018 Jun 28;9(6):663
pubmed: 29950557
Front Neural Circuits. 2017 Nov 06;11:84
pubmed: 29163065
J Physiol. 1987 Jul;388:183-98
pubmed: 3656190
Data Brief. 2018 Nov 06;21:2037-2041
pubmed: 30510990
Neuron. 2016 Aug 17;91(4):748-762
pubmed: 27499084
Sci Rep. 2018 Jan 15;8(1):742
pubmed: 29335507
J Exp Biol. 2021 Nov 1;224(21):
pubmed: 34632494
Front Cell Dev Biol. 2021 Nov 19;9:744191
pubmed: 34869332
Cells. 2021 Jun 06;10(6):
pubmed: 34204045
Dis Model Mech. 2019 Feb 20;12(2):
pubmed: 30709851
Neural Regen Res. 2022 Jul;17(7):1475-1477
pubmed: 34916423
Eur J Neurosci. 2012 Nov;36(10):3356-64
pubmed: 22882375
J Mol Neurosci. 2020 Sep;70(9):1345-1353
pubmed: 32406040
Neural Regen Res. 2020 Jun;15(6):996-1005
pubmed: 31823869
Neurotrauma Rep. 2022 Feb 14;3(1):15-26
pubmed: 35211695
J Physiol. 1978 Apr;277:395-408
pubmed: 650547
Biol Bull. 2020 Dec;239(3):174-182
pubmed: 33347797
J Cell Biol. 1985 Apr;100(4):1284-94
pubmed: 2579958
Sci Rep. 2016 Nov 25;6:37786
pubmed: 27886236
J Comp Neurol. 2009 Jul 20;515(3):295-312
pubmed: 19425080
J Comp Neurol. 2022 Sep 23;:
pubmed: 36150899
J Neurosci. 2021 Aug 25;41(34):7314-7325
pubmed: 34193553
Neural Regen Res. 2015 Jan;10(1):25-7
pubmed: 25788909
Front Cell Dev Biol. 2021 Mar 25;9:653638
pubmed: 33842481
Exp Neurol. 2013 Dec;250:31-42
pubmed: 24041988
J Neurosci. 2011 Apr 13;31(15):5605-16
pubmed: 21490201
Rev Neurol. 2012 Aug 1;55(3):157-66
pubmed: 22825976
J Chem Neuroanat. 2021 Dec;118:102041
pubmed: 34774721
J Comp Neurol. 1976 Aug 15;168(4):545-54
pubmed: 939822
Am J Physiol Regul Integr Comp Physiol. 2013 Jun 1;304(11):R940-50
pubmed: 23552576
Neural Regen Res. 2022 Oct;17(10):2272-2277
pubmed: 35259849
Neuropharmacology. 2018 Mar 15;131:389-402
pubmed: 29317225
Neural Regen Res. 2017 Apr;12(4):525-528
pubmed: 28553321
Exp Brain Res. 1993;97(1):83-95
pubmed: 8131834
Data Brief. 2020 Jan 03;28:105092
pubmed: 31956679
J Neurotrauma. 2011 Dec;28(12):2535-40
pubmed: 21568687
J Neurophysiol. 2021 Dec 1;126(6):1959-1977
pubmed: 34731061
Front Cell Dev Biol. 2021 Nov 26;9:774650
pubmed: 34901020