Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
20
01
2022
accepted:
03
09
2022
entrez:
2
11
2022
pubmed:
3
11
2022
medline:
5
11
2022
Statut:
epublish
Résumé
Madurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of M. mycetomatis and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that M. mycetomatis reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +/- 21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including Streptococcus sp., Staphylococcus sp. and others.
Identifiants
pubmed: 36322569
doi: 10.1371/journal.pntd.0010787
pii: PNTD-D-22-00051
pmc: PMC9629555
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0010787Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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