Impact of peptide permeation enhancer on tight junctions opening cellular mechanisms.
L-R5 peptide
Occludin
PKC zeta
Protein interaction
Tight junction
Journal
Biochemistry and biophysics reports
ISSN: 2405-5808
Titre abrégé: Biochem Biophys Rep
Pays: Netherlands
ID NLM: 101660999
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
20
07
2022
revised:
03
10
2022
accepted:
24
10
2022
entrez:
3
11
2022
pubmed:
4
11
2022
medline:
4
11
2022
Statut:
epublish
Résumé
The myristoylated pentapeptide, L-R5, contains an amino acid sequence of the zeta inhibitory peptide (ZIP) portion (pseudosubstrate) of protein kinase C zeta (PKC ζ). As PKC ζ is involved in the modulation of epithelial tight junctions (TJs) through the phosphorylation of TJ proteins, L-R5 was suggested to interact with the enzyme resulting in the enhancement of paracellular permeability. This study shows that L-R5 does not bind to the enzyme but interacts directly with TJ proteins. We show here that the binding of PKC ζ to occludin and its successive phosphorylation is prevented by L-R5, which leads to TJ disruption and enhanced epithelial permeability. Although L-R5 did not show any
Identifiants
pubmed: 36324528
doi: 10.1016/j.bbrep.2022.101375
pii: S2405-5808(22)00175-3
pmc: PMC9618981
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101375Informations de copyright
© 2022 The Authors. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Eur J Pharm Sci. 2016 Oct 10;93:405-9
pubmed: 27516147
Cell. 1993 Aug 13;74(3):555-63
pubmed: 7688666
Gastroenterology. 2004 Jul;127(1):224-38
pubmed: 15236188
Arch Biochem Biophys. 2017 May 1;621:24-30
pubmed: 28389298
Chem Rev. 2001 Aug;101(8):2353-64
pubmed: 11749377
Dev Cell. 2007 Nov;13(5):609-622
pubmed: 17981131
J Biol Chem. 1998 Nov 13;273(46):30306-15
pubmed: 9804792
Adv Sci (Weinh). 2021 Oct;8(19):e2100478
pubmed: 34382375
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6072-6
pubmed: 7597083
Physiol Rev. 2008 Oct;88(4):1341-78
pubmed: 18923184
Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10402-7
pubmed: 18641122
Alzheimers Res Ther. 2014 Mar 06;6(2):13
pubmed: 25031631
Am J Physiol Lung Cell Mol Physiol. 2000 Sep;279(3):L429-38
pubmed: 10956616
Adv Enzyme Regul. 2008;48:166-78
pubmed: 18167314
Semin Cell Dev Biol. 2015 Jun;42:47-57
pubmed: 25951797
J Neurosci. 2013 Jul 3;33(27):11012-22
pubmed: 23825406
Eur J Pharm Sci. 2021 May 1;160:105747
pubmed: 33582284
Stem Cells. 2015 Mar;33(3):819-32
pubmed: 25376707
Mol Cell. 2015 Apr 16;58(2):203-15
pubmed: 25818647
J Cell Mol Med. 2019 Jan;23(1):317-327
pubmed: 30450773
Cell. 2006 May 5;125(3):535-48
pubmed: 16678097
J Neurosci. 2005 Feb 23;25(8):1979-84
pubmed: 15728837
Science. 1987 Dec 18;238(4834):1726-8
pubmed: 3686012
Curr Chem Genomics. 2011;5:115-21
pubmed: 21966344
Nat Rev Mol Cell Biol. 2018 May;19(5):313-326
pubmed: 29410531
Mol Pharmacol. 2015 Oct;88(4):728-35
pubmed: 26199377
J Biol Chem. 2012 Apr 13;287(16):12879-85
pubmed: 22378786
Prog Neurobiol. 2006 Jul;79(4):173-89
pubmed: 16935409
Mol Cell. 2013 May 23;50(4):528-39
pubmed: 23665229
J Med Chem. 2014 Aug 14;57(15):6513-30
pubmed: 25058929
Oncotarget. 2017 Jun 27;8(39):65329-65338
pubmed: 29029434
Biochem J. 1993 Apr 15;291 ( Pt 2):329-43
pubmed: 8484714
Exp Cell Res. 2015 Feb 15;331(2):257-66
pubmed: 25592443
J Biol Chem. 2014 Sep 5;289(36):25021-30
pubmed: 25035426
Nature. 2013 Jan 17;493(7432):416-9
pubmed: 23283171
J Invest Dermatol. 2007 Apr;127(4):782-91
pubmed: 17110935
Biochem J. 2002 Dec 1;368(Pt 2):471-81
pubmed: 12169098
Annu Rev Biochem. 2003;72:609-42
pubmed: 12676795
Nat Cell Biol. 2009 Aug;11(8):1024-30
pubmed: 19620967
J Cell Biol. 2002 Sep 2;158(5):967-78
pubmed: 12196510
Am J Clin Pathol. 2001 Sep;116(3):377-85
pubmed: 11554166
Diabetologia. 2001 Jun;44(6):659-73
pubmed: 11440359
Cancer Res. 2006 Sep 15;66(18):9125-33
pubmed: 16982755
EMBO J. 1997 Sep 15;16(18):5662-71
pubmed: 9312025
J Biol Chem. 2001 Feb 16;276(7):4709-16
pubmed: 11085981
Adv Drug Deliv Rev. 2021 Apr;171:266-288
pubmed: 33617902
Life Sci. 2021 Jan 15;265:118860
pubmed: 33301813
Am J Physiol. 1997 Dec;273(6):C1859-67
pubmed: 9435490
J Biol Chem. 2015 Sep 4;290(36):21845-56
pubmed: 26187466
Methods. 2013 Mar;59(3):301-15
pubmed: 23270813
Semin Cell Dev Biol. 2014 Dec;36:166-76
pubmed: 25220018
Biochem J. 2011 Jul 15;437(2):289-99
pubmed: 21545357
Front Biosci (Landmark Ed). 2009 Jan 01;14(6):2386-99
pubmed: 19273207
Trends Endocrinol Metab. 2009 Jan;20(1):25-33
pubmed: 19056296
Cancer Cell. 2019 Sep 16;36(3):218-235
pubmed: 31474570
Ann N Y Acad Sci. 2009 May;1165:62-8
pubmed: 19538289
EMBO J. 2004 Nov 24;23(23):4595-605
pubmed: 15526032
J Biol Chem. 2007 Apr 13;282(15):11487-98
pubmed: 17298946
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5822-4
pubmed: 3526339
Oncogene. 2008 Nov 24;27(55):6878-87
pubmed: 19029931
Elife. 2020 Aug 06;9:
pubmed: 32758356
Front Cell Dev Biol. 2022 Jul 13;10:911279
pubmed: 35912112
Biochem J. 1989 Oct 15;263(2):597-9
pubmed: 2597123