Oropharyngeal samples versus lesion specimens at diagnosis in patients infected with monkeypox virus in Northern France.
epidemiology
infection
poxvirus
viral excretion
virus classification
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
revised:
22
10
2022
received:
12
09
2022
accepted:
31
10
2022
pubmed:
4
11
2022
medline:
11
1
2023
entrez:
3
11
2022
Statut:
ppublish
Résumé
The ongoing outbreak of monkeypox virus (MPXV) is the largest one in historically non-endemic countries. Early reports described atypical epidemiological and clinical presentations. We investigated MPXV DNA detection in oropharyngeal samples (OPS), and compared the viral load to that in lesion samples at diagnosis in patients infected with MPXV. We retrospectively included patients suspected to have monkeypox in Northern France, who underwent a MPXV PCR in the Virology Laboratory, University Hospital of Lille, from May 23 to August 18, 2022. Overall, a total of 228 patients (376 samples) were included. A positive result in at least one sample was found in 138 patients (60.5%). We compared PCR results between OPS and lesion samples (i.e., cutaneous or anal/rectal samples) in patients with both samples. A positive result in OPS was observed in 54 out of 60 patients (90%). The viral load in OPS (median C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e28276Informations de copyright
© 2022 Wiley Periodicals LLC.
Références
Vivancos R, Anderson C, Blomquist P, et al. Community transmission of monkeypox in the United Kingdom, April to May 2022. Euro Surveill. 2022;27(22):2200422. doi:10.2807/1560-7917.ES.2022.27.22.2200422
Kluge H, Ammon A. Monkeypox in Europe and beyond-tackling a neglected disease together. Euro Surveill. 2022;27(24):2200482. doi:10.2807/1560-7917.ES.2022.27.24.2200482
Nuzzo JB, Borio LL, Gostin LO. The WHO declaration of monkeypox as a global public health emergency. JAMA. 2022;328(7):615. doi:10.1001/jama.2022.12513
CDC. Monkeypox outbreak global map. 2022. Accessed August 19, 2022. https://www.cdc.gov/poxvirus/monkeypox/response/2022/world-map.html
ECDC. Monkeypox multi-country outbreak. 2022. Accessed August 19, 2022. https://www.ecdc.europa.eu/en/monkeypox-outbreak
WHO. Monkeypox. 2022. Accessed August 16, 2022. https://www.who.int/news-room/fact-sheets/detail/monkeypox
Lapa D, Carletti F, Mazzotta V, et al. Monkeypox virus isolation from a semen sample collected in the early phase of infection in a patient with prolonged seminal viral shedding. Lancet Infect Dis. 2022;22:1267-1269. doi:10.1016/S1473-3099(22)00513-8
Del Rio C, Malani PN. Update on the monkeypox outbreak. JAMA. 2022;328:921. doi:10.1001/jama.2022.14857
von Elm E, Altman DG, Egger M, Pocock SJ, Gøtzsche PC, Vandenbroucke JP. The strengthening the reporting of observational studies in epidemiology (STROBE) statement: guidelines for reporting observational studies. Lancet. 2007;370(9596):1453-1457. doi:10.1016/S0140-6736(07)61602-X
Benchimol EI, Smeeth L, Guttmann A, et al. The REporting of studies conducted using observational routinely-collected health data (RECORD) statement. PLoS Med. 2015;12(10):e1001885. doi:10.1371/journal.pmed.1001885
Li Y, Zhao H, Wilkins K, Hughes C, Damon IK. Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA. J Virol Methods. 2010;169(1):223-227. doi:10.1016/j.jviromet.2010.07.012
Girometti N, Byrne R, Bracchi M, et al. Demographic and clinical characteristics of confirmed human monkeypox virus cases in individuals attending a sexual health centre in London, UK: an observational analysis. Lancet Infect Dis. 2022;22:1321-1328. doi:10.1016/S1473-3099(22)00411-X
Thornhill JP, Barkati S, Walmsley S, et al. Monkeypox virus infection in humans across 16 countries-April-June 2022. N Engl J Med. 2022;387:679-691. doi:10.1056/NEJMoa2207323
Patel A, Bilinska J, Tam JCH, et al. Clinical features and novel presentations of human monkeypox in a central London centre during the 2022 outbreak: descriptive case series. BMJ. 2022;378:e072410. doi:10.1136/bmj-2022-072410
Tarín-Vicente EJ, Alemany A, Agud-Dios M, et al. Clinical presentation and virological assessment of confirmed human monkeypox virus cases in Spain: a prospective observational cohort study. Lancet. 2022;400:661-669. doi:10.1016/S0140-6736(22)01436-2
Adler H, Gould S, Hine P, et al. Clinical features and management of human monkeypox: a retrospective observational study in the UK. Lancet Infect Dis. 2022;22(8):1153-1162. doi:10.1016/S1473-3099(22)00228-6