Notch activation during early mesoderm induction modulates emergence of the T/NK cell lineage from human iPSCs.
T cell
hematopoietic progenitor
human
iPSC
natural killer cell
notch
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
13 12 2022
13 12 2022
Historique:
received:
16
11
2021
revised:
06
10
2022
accepted:
07
10
2022
pubmed:
5
11
2022
medline:
17
12
2022
entrez:
4
11
2022
Statut:
ppublish
Résumé
A robust method of producing mature T cells from iPSCs is needed to realize their therapeutic potential. NOTCH1 is known to be required for the production of hematopoietic progenitor cells with T cell potential in vivo. Here we identify a critical window during mesodermal differentiation when Notch activation robustly improves access to definitive hematopoietic progenitors with T/NK cell lineage potential. Low-density progenitors on either OP9-hDLL4 feeder cells or hDLL4-coated plates favored T cell maturation into TCRab
Identifiants
pubmed: 36332629
pii: S2213-6711(22)00503-3
doi: 10.1016/j.stemcr.2022.10.007
pmc: PMC9768581
pii:
doi:
Substances chimiques
Receptors, Notch
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2610-2628Subventions
Organisme : NHLBI NIH HHS
ID : F32 HL149605
Pays : United States
Organisme : NHLBI NIH HHS
ID : 75N92020C00005
Pays : United States
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interests Boston Medical Center has filed a patent application on the findings of this manuscript. G. Mostoslavsky is a scientific founder of and holds equity in Clade Therapeutics.
Références
J Exp Med. 2017 Nov 6;214(11):3347-3360
pubmed: 28931624
J Clin Invest. 1990 Mar;85(3):904-15
pubmed: 2312733
Nat Commun. 2021 Jan 18;12(1):430
pubmed: 33462228
Cell Stem Cell. 2018 Aug 02;23(2):252-265.e8
pubmed: 30082068
Blood. 2009 Feb 19;113(8):1689-98
pubmed: 19001083
J Exp Med. 2005 May 2;201(9):1361-6
pubmed: 15851488
Blood. 2012 Jun 14;119(24):5706-14
pubmed: 22431573
Cell Stem Cell. 2013 Nov 7;13(5):535-48
pubmed: 24054998
Trends Immunol. 2003 Apr;24(4):155-8
pubmed: 12697438
Methods. 2016 May 15;101:65-72
pubmed: 26439174
Dev Dyn. 2015 Mar;244(3):391-409
pubmed: 25641373
Immunity. 2017 Jul 18;47(1):183-198.e6
pubmed: 28723550
Nat Biotechnol. 2013 Oct;31(10):928-33
pubmed: 23934177
Cell Stem Cell. 2014 Sep 4;15(3):350-364
pubmed: 25130491
Blood. 1992 Feb 1;79(3):666-77
pubmed: 1370641
Nature. 2019 Oct;574(7778):365-371
pubmed: 31597962
Dev Cell. 2017 Mar 13;40(5):505-511.e6
pubmed: 28292428
J Immunol. 2014 May 1;192(9):4017-23
pubmed: 24748636
Cell Stem Cell. 2018 Dec 6;23(6):850-858.e4
pubmed: 30449714
Mech Dev. 2009 May-Jun;126(5-6):314-23
pubmed: 19275935
Eur J Cell Biol. 2018 Sep;97(7):512-522
pubmed: 30249464
BMC Bioinformatics. 2013 Apr 15;14:128
pubmed: 23586463
Mol Cell Biol. 2004 Oct;24(20):8813-22
pubmed: 15456857
Nat Immunol. 2004 Apr;5(4):410-7
pubmed: 15034575
Blood. 2005 Dec 1;106(12):3988-94
pubmed: 16091451
Nature. 2021 Dec;600(7888):285-289
pubmed: 34789876
Immunity. 2019 Nov 19;51(5):930-948.e6
pubmed: 31604687
Cell. 2015 Dec 17;163(7):1663-77
pubmed: 26627738
Int Immunol. 1999 Aug;11(8):1195-202
pubmed: 10421777
Blood. 2004 Nov 15;104(10):3097-105
pubmed: 15251982
Blood. 2007 May 15;109(10):4478-86
pubmed: 17272511
Nat Cell Biol. 2015 May;17(5):580-91
pubmed: 25915127
Cell Stem Cell. 2019 Mar 7;24(3):376-389.e8
pubmed: 30661959
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Stem Cells. 2018 Jul;36(7):1004-1019
pubmed: 29569827
Nat Rev Mol Cell Biol. 2016 Nov;17(11):722-735
pubmed: 27507209
BMC Immunol. 2011 Mar 23;12:22
pubmed: 21429219
Nature. 2011 Jun 08;474(7350):220-4
pubmed: 21654806
Dev Cell. 2015 Jun 22;33(6):729-36
pubmed: 26051539
Blood. 2006 Jul 1;108(1):116-22
pubmed: 16339407
Immunity. 2003 May;18(5):699-711
pubmed: 12753746
Nat Commun. 2018 May 8;9(1):1828
pubmed: 29739946