Single cell profiling of CD45
B cells
Cell–cell communication
Ectopic lymphoid follicle
Microglia
Single cell RNA-sequencing
Spinal cord injury
Journal
Journal of neuroinflammation
ISSN: 1742-2094
Titre abrégé: J Neuroinflammation
Pays: England
ID NLM: 101222974
Informations de publication
Date de publication:
04 Nov 2022
04 Nov 2022
Historique:
received:
08
04
2022
accepted:
20
10
2022
entrez:
5
11
2022
pubmed:
6
11
2022
medline:
9
11
2022
Statut:
epublish
Résumé
Immune cells play crucial roles after spinal cord injury (SCI). However, incomplete knowledge of immune contributions to injury and repair hinders development of SCI therapies. We leveraged single-cell observations to describe key populations of immune cells present in the spinal cord and changes in their transcriptional profiles from uninjured to subacute and chronic stages of SCI. Deep-read single-cell sequencing was performed on CD45 In uninjured and 7 dpi spinal cord, most CD45 Immune cell responses to SCI have region-specific aspects and evolve with time. Developmentally diverse populations of B cells accumulate in the spinal cord following injury. Microglia at subacute stages express B cell recruitment factors, while chronically, they express factors predicted to reduce B cell inflammatory state. In the injured spinal cord, B cells create ectopic lymphoid structures, and express secreted factors potentially acting on microglia. Our study predicts previously unidentified crosstalk between microglia and B cells post-injury at acute and chronic stages, revealing new potential targets of inflammatory responses for SCI repair warranting future functional analyses.
Sections du résumé
BACKGROUND
BACKGROUND
Immune cells play crucial roles after spinal cord injury (SCI). However, incomplete knowledge of immune contributions to injury and repair hinders development of SCI therapies. We leveraged single-cell observations to describe key populations of immune cells present in the spinal cord and changes in their transcriptional profiles from uninjured to subacute and chronic stages of SCI.
METHODS
METHODS
Deep-read single-cell sequencing was performed on CD45
RESULTS
RESULTS
In uninjured and 7 dpi spinal cord, most CD45
CONCLUSIONS
CONCLUSIONS
Immune cell responses to SCI have region-specific aspects and evolve with time. Developmentally diverse populations of B cells accumulate in the spinal cord following injury. Microglia at subacute stages express B cell recruitment factors, while chronically, they express factors predicted to reduce B cell inflammatory state. In the injured spinal cord, B cells create ectopic lymphoid structures, and express secreted factors potentially acting on microglia. Our study predicts previously unidentified crosstalk between microglia and B cells post-injury at acute and chronic stages, revealing new potential targets of inflammatory responses for SCI repair warranting future functional analyses.
Identifiants
pubmed: 36333772
doi: 10.1186/s12974-022-02627-3
pii: 10.1186/s12974-022-02627-3
pmc: PMC9635187
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
266Subventions
Organisme : New York State Department of Health
ID : C34724GG
Organisme : New York State Department of Health
ID : C32244GG
Informations de copyright
© 2022. The Author(s).
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