Optimisation of Calophyllum inophyllum seed oil nanoemulsion as a potential wound healing agent.

Calophyllum / chemistry Emulsions / pharmacology Wound Healing Plant Oils / pharmacology Water
Cell viability Nanoemulsion RSM Wound healing

Journal

BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232

Informations de publication

Date de publication:
04 Nov 2022
Historique:
received: 27 04 2022
accepted: 05 10 2022
entrez: 5 11 2022
pubmed: 6 11 2022
medline: 9 11 2022
Statut: epublish

Résumé

Efficient delivery systems of Calophyllum inophyllum seed oil (CSO) in the form of nanoemulsion were optimised to enhance its stability and ensure its therapeutic efficiency as a potential agent for various biomedical applications. Response Surface Methodology (RSM) was used to determine the effects of independent variables (oil, surfactant, water percentage and homogenisation time) on physicochemical characteristics, including droplet size, polydispersity index and turbidity. The optimised CSO nanoemulsion (CSONE) has a 46.68 nm particle size, 0.15 Polydispersity index value and 1.16 turbidity. After 4 weeks of storage at 5 ± 1 °C and 25 ± 1 °C, the CSONE was physically stable. The optimised CSO nanoemulsion showed enhancement in cell viability and wound healing in baby hamster kidney a clone BHK-21 (BSR) cells as compared to the CSO. The wound healing property of CSONE was higher than CSO. Thus, our in vitro wound healing results demonstrated that CSO in the nanoemulsion form can promote wound healing by enhancing the proliferation and migration of epidermal cells. The coarse emulsion of Calophyllum inophyllum seed oil nano emulsion was prepared using high shear homogeniser techniques. The optimised CSONE with the droplet size of 46.68 nm was prepared from a mixture of CSO, Tween 80, and high pure water (HPW), then used for the biological investigation. The in vitro cell monolayer scratch assay revealed that CSONE in the lowest concentration of CSO resulted in 100% wound closure after 48 hrs. The optimised CSO nanoemulsion was found to be a promising and effective approach in the treatment of wounds by boosting the proliferation and migration of epidermal cells.

Sections du résumé

BACKGROUND BACKGROUND
Efficient delivery systems of Calophyllum inophyllum seed oil (CSO) in the form of nanoemulsion were optimised to enhance its stability and ensure its therapeutic efficiency as a potential agent for various biomedical applications.
METHOD METHODS
Response Surface Methodology (RSM) was used to determine the effects of independent variables (oil, surfactant, water percentage and homogenisation time) on physicochemical characteristics, including droplet size, polydispersity index and turbidity.
RESULTS RESULTS
The optimised CSO nanoemulsion (CSONE) has a 46.68 nm particle size, 0.15 Polydispersity index value and 1.16 turbidity. After 4 weeks of storage at 5 ± 1 °C and 25 ± 1 °C, the CSONE was physically stable. The optimised CSO nanoemulsion showed enhancement in cell viability and wound healing in baby hamster kidney a clone BHK-21 (BSR) cells as compared to the CSO. The wound healing property of CSONE was higher than CSO.
CONCLUSION CONCLUSIONS
Thus, our in vitro wound healing results demonstrated that CSO in the nanoemulsion form can promote wound healing by enhancing the proliferation and migration of epidermal cells. The coarse emulsion of Calophyllum inophyllum seed oil nano emulsion was prepared using high shear homogeniser techniques. The optimised CSONE with the droplet size of 46.68 nm was prepared from a mixture of CSO, Tween 80, and high pure water (HPW), then used for the biological investigation. The in vitro cell monolayer scratch assay revealed that CSONE in the lowest concentration of CSO resulted in 100% wound closure after 48 hrs. The optimised CSO nanoemulsion was found to be a promising and effective approach in the treatment of wounds by boosting the proliferation and migration of epidermal cells.

Identifiants

DOI: 10.1186/s12906-022-03751-6 PMID: 36333789 PMC: PMC9635111
pubmed: 36333789
doi: 10.1186/s12906-022-03751-6
pii: 10.1186/s12906-022-03751-6
pmc: PMC9635111
doi:

Substances chimiques

Emulsions 0
Plant Oils 0
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

285

Subventions

Organisme : Charles Darwin University
ID : PhD researcher

Informations de copyright

© 2022. The Author(s).

Références

Life Sci. 2020 Mar 1;244:117324
pubmed: 31958420
Int Wound J. 2017 Feb;14(1):89-96
pubmed: 26688157
PLoS One. 2015 Sep 25;10(9):e0138602
pubmed: 26406588
Planta Med. 2016 Jul;82(11-12):961-6
pubmed: 27280931
Clin Dermatol. 1994 Jan-Mar;12(1):157-69
pubmed: 8180938
Int J Nanomedicine. 2022 Jul 06;17:2995-3012
pubmed: 35832117
Colloids Surf B Biointerfaces. 2018 Dec 1;172:170-179
pubmed: 30172200
Food Chem. 2018 Feb 1;240:1146-1153
pubmed: 28946236
Int J Biol Macromol. 2016 Mar;84:261-7
pubmed: 26708427
Colloids Surf B Biointerfaces. 2013 Dec 1;112:113-9
pubmed: 23974000
Mutat Res. 2013 Jan-Mar;752(1):10-24
pubmed: 22940039
3 Biotech. 2015 Apr;5(2):123-127
pubmed: 28324579
Physiol Rev. 2019 Jan 1;99(1):665-706
pubmed: 30475656
Fitoterapia. 2010 Sep;81(6):586-9
pubmed: 20188156
PLoS One. 2017 Oct 11;12(10):e0185674
pubmed: 29020015
Contemp Clin Dent. 2019 Apr-Jun;10(2):319-323
pubmed: 32308297
J Ethnopharmacol. 2009 Jun 25;123(3):452-8
pubmed: 19501278
Pharmaceutics. 2017 Sep 21;9(4):
pubmed: 28934172
PeerJ. 2019 May 22;7:e6896
pubmed: 31198623
Wound Repair Regen. 2004 Mar-Apr;12(2):235-43
pubmed: 15086775
Nutr Cancer. 2022;74(6):2196-2206
pubmed: 34607477
Med Res Rev. 2002 Jan;22(1):65-75
pubmed: 11746176
Int J Nanomedicine. 2015 Feb 04;10:1109-18
pubmed: 25709435
Eur J Pharm Sci. 2012 Oct 9;47(3):564-73
pubmed: 22841998
Colloids Surf B Biointerfaces. 2014 Jan 1;113:330-7
pubmed: 24121076
J Invest Dermatol. 1983 Jun;80 Suppl:44s-49s
pubmed: 6189923
Indian J Med Res. 1980 Nov;72:762-5
pubmed: 7203581
Food Chem. 2018 Jul 1;253:179-184
pubmed: 29502819
Bioorg Med Chem Lett. 1998 Dec 15;8(24):3475-8
pubmed: 9934455
Crit Rev Food Sci Nutr. 2011 Apr;51(4):285-330
pubmed: 21432697
Food Chem. 2019 Sep 30;293:134-143
pubmed: 31151593
J Nat Prod. 2000 Nov;63(11):1471-4
pubmed: 11087585
Int J Pharm. 2020 Apr 30;580:119209
pubmed: 32165223
J Biotechnol. 2007 Jul 15;130(4):346-53
pubmed: 17601621
J Control Release. 2017 Feb 10;247:86-105
pubmed: 28024914

Auteurs

Elnaz Saki (E)

College of Engineering, IT & Environment, Charles Darwin University, Casuarina campus, Darwin City, 0810, Australia. Elnaz.saki@cdu.edu.au.
ORCID: 0000-0001-7707-2617

Vinuthaa Murthy (V)

College of Engineering, IT & Environment, Charles Darwin University, Casuarina campus, Darwin City, 0810, Australia. Vinuthaa.murthy@cdu.edu.au.

Roshanak Khandanlou (R)

College of Engineering, IT & Environment, Charles Darwin University, Casuarina campus, Darwin City, 0810, Australia.

Hao Wang (H)

College of Engineering, IT & Environment, Charles Darwin University, Casuarina campus, Darwin City, 0810, Australia.

Johanna Wapling (J)

Menzies School of Health Research, Charles Darwin University, Darwin City, Australia.

Richard Weir (R)

Berrimah Veterinary Laboratory, Department of Industry, Tourism and Trade Northern Territory Government, Darwin City, Australia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Calophyllum › Optimisation of Calophyllum inophyllum seed...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Émulsions › Optimisation of Calophyllum inophyllum seed...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Cicatrisation de plaie › Optimisation of Calophyllum inophyllum seed...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Préparations à base de plantes › Huiles végétales › Optimisation of Calophyllum inophyllum seed...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Eau › Optimisation of Calophyllum inophyllum seed...