Identification of Babesia bovis MSA-1 functionally constraint regions capable of binding to bovine erythrocytes.
Babesia bovis
Bovine erythrocyte
High activity binding peptides
MSA-1
Parasite adhesion
Synthetic vaccine
Journal
Veterinary parasitology
ISSN: 1873-2550
Titre abrégé: Vet Parasitol
Pays: Netherlands
ID NLM: 7602745
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
19
05
2022
revised:
23
09
2022
accepted:
19
10
2022
pubmed:
8
11
2022
medline:
6
12
2022
entrez:
7
11
2022
Statut:
ppublish
Résumé
Merozoite surface antigen-1 is a glycoprotein expressed by Babesia bovis and is considered a vaccine candidate given that antibodies against it are able to partially block in vitro invasion of bovine erythrocytes. Despite this, no study to date has confirmed the target cell binding properties of the full MSA-1 or its fragments. This research has thus been focused on identifying protein regions playing a role in erythrocyte attachment, based on genetic diversity and natural selection analysis. Two regions under functional constraint (nucleotides 134-428 and 464-629) having a preponderance of negatively-selected signals were identified in silico. Three non-overlapping peptides derived from functionally constraint regions (42422 (
Identifiants
pubmed: 36343530
pii: S0304-4017(22)00188-1
doi: 10.1016/j.vetpar.2022.109834
pii:
doi:
Substances chimiques
Merozoite Surface Protein 1
0
Antigens, Protozoan
0
Protozoan Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109834Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.