Targeted Suppression of Peptide Degradation in Ag-Based Surface-Enhanced Raman Spectra by Depletion of Hot Carriers.

Ag-surface-enhanced Raman spectroscopy (SERS) Kelvin probe force microscopy hot carriers iodides peptides sample degradation

Journal

Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338

Informations de publication

Date de publication:
12 2022
Historique:
revised: 10 10 2022
received: 18 08 2022
pubmed: 8 11 2022
medline: 31 12 2022
entrez: 7 11 2022
Statut: ppublish

Résumé

Sample degradation, in particular of biomolecules, frequently occurs in surface-enhanced Raman spectroscopy (SERS) utilizing supported silver SERS substrates. Currently, thermal and/or photocatalytic effects are considered to cause sample degradation. This paper establishes the efficient inhibition of sample degradation using iodide which is demonstrated by a systematic SERS study of a small peptide in aqueous solution. Remarkably, a distinct charge separation-induced surface potential difference is observed for SERS substrates under laser irradiation using Kelvin probe force microscopy. This directly unveils the photocatalytic effect of Ag-SERS substrates. Based on the presented results, it is proposed that plasmonic photocatalysis dominates sample degradation in SERS experiments and the suppression of typical SERS sample degradation by iodide is discussed by means of the energy levels of the substrate under mild irradiation conditions. This approach paves the way toward more reliable and reproducible SERS studies of biomolecules under physiological conditions.

Identifiants

pubmed: 36344458
doi: 10.1002/smll.202205080
doi:

Substances chimiques

Iodides 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2205080

Informations de copyright

© 2022 The Authors. Small published by Wiley-VCH GmbH.

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Auteurs

Xiaobin Yao (X)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.
Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena, Lessingstr. 10, 07743, Jena, Germany.

Christiane Höppener (C)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.
Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena, Lessingstr. 10, 07743, Jena, Germany.

Henrik Schneidewind (H)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.

Stephanie Hoeppener (S)

Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.
Laboratory of Organic and Macromolecular Chemistry, Friedrich Schiller University Jena, Humboldtstr. 10, 07743, Jena, Germany.

Zian Tang (Z)

Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena, Lessingstr. 10, 07743, Jena, Germany.

Axel Buchholz (A)

Institute of Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, 07743, Jena, Germany.

Annika König (A)

Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Beutenbergstrasse 11a, 07745, Jena, Germany.

Selene Mogavero (S)

Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Beutenbergstrasse 11a, 07745, Jena, Germany.

Marco Diegel (M)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.

Jan Dellith (J)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.

Andrey Turchanin (A)

Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena, Lessingstr. 10, 07743, Jena, Germany.

Winfried Plass (W)

Institute of Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, 07743, Jena, Germany.

Bernhard Hube (B)

Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Beutenbergstrasse 11a, 07745, Jena, Germany.
Institute of Microbiology, Friedrich Schiller University Jena, Neugasse 25, 07743, Jena, Germany.

Volker Deckert (V)

Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Str. 9, 07745, Jena, Germany.
Institute of Physical Chemistry (IPC) and Abbe Center of Photonics, Friedrich Schiller University Jena, Lessingstr. 10, 07743, Jena, Germany.
Institute For Quantum Science and Engineering (IQSE), Texas A&M University, College Station, TX, 77843, USA.

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