LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation.
LRG1
adipokine
cell biology
cytochrome c
insulin resistance
mouse
obesity
secretome
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
08 11 2022
08 11 2022
Historique:
received:
02
07
2022
accepted:
06
11
2022
pubmed:
9
11
2022
medline:
23
11
2022
entrez:
8
11
2022
Statut:
epublish
Résumé
While dysregulation of adipocyte endocrine function plays a central role in obesity and its complications, the vast majority of adipokines remain uncharacterized. We employed bio-orthogonal non-canonical amino acid tagging (BONCAT) and mass spectrometry to comprehensively characterize the secretome of murine visceral and subcutaneous white and interscapular brown adip ocytes. Over 600 proteins were identified, the majority of which showed cell type-specific enrichment. We here describe a metabolic role for leucine-rich α-2 glycoprotein 1 (LRG1) as an obesity-regulated adipokine secreted by mature adipocytes. LRG1 overexpression significantly improved glucose homeostasis in diet-induced and genetically obese mice. This was associated with markedly reduced white adipose tissue macrophage accumulation and systemic inflammation. Mechanistically, we found LRG1 binds cytochrome
Identifiants
pubmed: 36346018
doi: 10.7554/eLife.81559
pii: 81559
pmc: PMC9674348
doi:
pii:
Substances chimiques
Adipokines
0
Insulin
0
LRG1 protein, mouse
0
Glycoproteins
0
Banques de données
GEO
['GSE208219', 'GSE36026', 'GSE1133']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDDK NIH HHS
ID : RC2 DK129961
Pays : United States
Informations de copyright
© 2022, Choi et al.
Déclaration de conflit d'intérêts
CC, WB, SZ, CM, AP, MK, ZL, SS, FM, AC, TC, HM, PC No competing interests declared
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