Application of shear stress for enhanced osteogenic differentiation of mouse induced pluripotent stem cells.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 11 2022
08 11 2022
Historique:
received:
01
07
2022
accepted:
27
09
2022
entrez:
8
11
2022
pubmed:
9
11
2022
medline:
11
11
2022
Statut:
epublish
Résumé
The self-organizing potential of induced pluripotent stem cells (iPSCs) represents a promising tool for bone tissue engineering. Shear stress promotes the osteogenic differentiation of mesenchymal stem cells, leading us to hypothesize that specific shear stress could enhance the osteogenic differentiation of iPSCs. For osteogenesis, embryoid bodies were formed for two days and then maintained in medium supplemented with retinoic acid for three days, followed by adherent culture in osteogenic induction medium for one day. The cells were then subjected to shear loading (0.15, 0.5, or 1.5 Pa) for two days. Among different magnitudes tested, 0.5 Pa induced the highest levels of osteogenic gene expression and greatest mineral deposition, corresponding to upregulated connexin 43 (Cx43) and phosphorylated Erk1/2 expression. Erk1/2 inhibition during shear loading resulted in decreased osteogenic gene expression and the suppression of mineral deposition. These results suggest that shear stress (0.5 Pa) enhances the osteogenic differentiation of iPSCs, partly through Cx43 and Erk1/2 signaling. Our findings shed light on the application of shear-stress technology to improve iPSC-based tissue-engineered bone for regenerative bone therapy.
Identifiants
pubmed: 36347883
doi: 10.1038/s41598-022-21479-8
pii: 10.1038/s41598-022-21479-8
pmc: PMC9643422
doi:
Substances chimiques
Connexin 43
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19021Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP21K17005
Organisme : the NSRF via the Program Management Unit for Human Resources & Institutional Development, Research and Innovation
ID : B16F640118
Informations de copyright
© 2022. The Author(s).
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