Downstream bioprocessing of human pluripotent stem cell-derived therapeutics.
bioprocessing
downstream
pluripotent stem cell
separation
Journal
Engineering in life sciences
ISSN: 1618-0240
Titre abrégé: Eng Life Sci
Pays: Germany
ID NLM: 101193313
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
received:
14
04
2021
revised:
08
07
2021
accepted:
16
08
2021
entrez:
9
11
2022
pubmed:
1
9
2021
medline:
1
9
2021
Statut:
epublish
Résumé
With the advancement in lineage-specific differentiation from human pluripotent stem cells (hPSCs), downstream cell separation has now become a critical step to produce hPSC-derived products. Since differentiation procedures usually result in a heterogeneous cell population, cell separation needs to be performed either to enrich the desired cell population or remove the undesired cell population. This article summarizes recent advances in separation processes for hPSC-derived cells, including the standard separation technologies, such as magnetic-activated cell sorting, as well as the novel separation strategies, such as those based on adhesion strength and metabolic flux. Specifically, the downstream bioprocessing flow and the identification of surface markers for various cell lineages are discussed. While challenges remain for large-scale downstream bioprocessing of hPSC-derived cells, the rational quality-by-design approach should be implemented to enhance the understanding of the relationship between process and the product and to ensure the safety of the produced cells.
Identifiants
pubmed: 36348655
doi: 10.1002/elsc.202100042
pii: ELSC1437
pmc: PMC9635003
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
667-680Informations de copyright
© 2021 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Stem Cells. 2007 Sep;25(9):2257-68
pubmed: 17588935
Cell Stem Cell. 2011 Mar 4;8(3):335-46
pubmed: 21362573
Stem Cells Transl Med. 2014 Dec;3(12):1473-83
pubmed: 25355733
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Biochem Biophys Res Commun. 2018 Jan 1;495(1):1278-1284
pubmed: 29175323
Cell Stem Cell. 2014 Jan 2;14(1):13-26
pubmed: 24388173
J Hepatol. 2013 Mar;58(3):625-9
pubmed: 23131523
PLoS One. 2012;7(11):e49874
pubmed: 23209609
Nature. 2008 May 22;453(7194):524-8
pubmed: 18432194
Cell Stem Cell. 2013 Jan 3;12(1):127-37
pubmed: 23168164
Tissue Eng Part B Rev. 2020 Aug;26(4):367-382
pubmed: 32571167
Differentiation. 2016 Apr-Jun;91(4-5):126-38
pubmed: 26915912
Nat Biotechnol. 2009 Jan;27(1):26-34
pubmed: 19131992
Stem Cell Reports. 2015 May 12;4(5):811-20
pubmed: 25866158
Methods. 2018 Jan 15;133:65-80
pubmed: 29037816
Cell Stem Cell. 2013 Jun 6;12(6):669-77
pubmed: 23746976
Nat Biotechnol. 2011 Jul 31;29(8):750-6
pubmed: 21804561
Biores Open Access. 2014 Aug 1;3(4):137-49
pubmed: 25126478
Nat Rev Genet. 2014 Feb;15(2):82-92
pubmed: 24434846
Stem Cell Reports. 2017 Nov 14;9(5):1406-1414
pubmed: 28988990
Langmuir. 2017 Feb 14;33(6):1576-1582
pubmed: 28092949
Stem Cell Res. 2009 Mar;2(2):113-24
pubmed: 19383417
Trends Biotechnol. 2009 Sep;27(9):546-53
pubmed: 19647883
Stem Cell Reports. 2017 Jul 11;9(1):12-22
pubmed: 28528701
Stem Cells. 2011 Apr;29(4):609-17
pubmed: 21308865
Biotechnol Adv. 2013 Dec;31(8):1600-23
pubmed: 23962714
Cell Stem Cell. 2011 Feb 4;8(2):228-40
pubmed: 21295278
PLoS One. 2015 Aug 31;10(8):e0135880
pubmed: 26323090
J Vis Exp. 2016 Mar 03;(109):
pubmed: 26966833
Mol Cell Neurosci. 2015 Sep;68:244-57
pubmed: 26284979
Stem Cell Res Ther. 2017 Sep 29;8(1):202
pubmed: 28962583
Stem Cells. 2009 Jun;27(6):1298-308
pubmed: 19489095
Commun Biol. 2018 Dec 7;1:218
pubmed: 30534610
Tissue Eng Part B Rev. 2020 Oct;26(5):402-422
pubmed: 32220216
Stem Cell Res Ther. 2020 Feb 3;11(1):47
pubmed: 32014053
Front Endocrinol (Lausanne). 2017 Oct 09;8:253
pubmed: 29067001
Cell Stem Cell. 2015 Jun 4;16(6):699-711
pubmed: 26004781
Sci Rep. 2016 Jan 05;6:18759
pubmed: 26729410
Circ Res. 2002 Sep 20;91(6):501-8
pubmed: 12242268
Stem Cell Rev Rep. 2009 Mar;5(1):72-80
pubmed: 19184635
Cell Rep. 2017 Mar 7;18(10):2533-2546
pubmed: 28273465
Stem Cell Res. 2010 Sep;5(2):91-103
pubmed: 20538536
Biofabrication. 2016 Sep 08;8(3):035017
pubmed: 27606680
Biomaterials. 2019 Nov;222:119431
pubmed: 31450160
Stem Cells Dev. 2012 Jul 1;21(10):1653-61
pubmed: 22221271
Development. 2014 Feb;141(4):784-94
pubmed: 24496616
PLoS One. 2017 May 11;12(5):e0173222
pubmed: 28493867
Development. 2016 May 1;143(9):1475-81
pubmed: 27143754
Nat Commun. 2016 Oct 14;7:13097
pubmed: 27739432
Stem Cells Transl Med. 2017 Apr;6(4):1158-1167
pubmed: 28213970
Stem Cells. 2008 Jun;26(6):1454-63
pubmed: 18356574
Biotechnol Bioeng. 2012 Nov;109(11):2699-709
pubmed: 22887094
Cell Stem Cell. 2019 May 2;24(5):802-811.e5
pubmed: 30880024
Stem Cell Reports. 2014 Mar 06;2(3):337-50
pubmed: 24672756
BMC Biotechnol. 2009 Apr 02;9:30
pubmed: 19341452
Stem Cells Transl Med. 2015 Oct;4(10):1155-63
pubmed: 26315572
Stem Cells Dev. 2013 Sep 15;22(18):2459-76
pubmed: 23621561
Mol Cell Proteomics. 2018 Sep;17(9):1670-1684
pubmed: 29848781
PLoS One. 2011 Mar 02;6(3):e17540
pubmed: 21407814
Nat Commun. 2013;4:1335
pubmed: 23299885
Stem Cells Dev. 2013 May 15;22(10):1497-505
pubmed: 23249362
Regen Med. 2017 Apr;12(4):397-417
pubmed: 28621179
Ann Biomed Eng. 2006 Jan;34(1):39-50
pubmed: 16463087
Biotechnol Adv. 2013 Dec;31(8):1374-85
pubmed: 23747736
Sci Rep. 2018 Apr 23;8(1):6375
pubmed: 29686294
PLoS One. 2019 Mar 27;14(3):e0213374
pubmed: 30917153
Mol Biotechnol. 2018 Nov;60(11):843-861
pubmed: 30196389
Circulation. 2013 Oct 22;128(17):1897-909
pubmed: 23995537
PLoS One. 2016 Jul 06;11(7):e0158282
pubmed: 27385038
Biomaterials. 2015 May;50:176-85
pubmed: 25736507
Stem Cells Dev. 2009 Mar;18(2):269-82
pubmed: 18651819
Sci Rep. 2018 Jan 16;8(1):804
pubmed: 29339826
PLoS One. 2011;6(8):e23657
pubmed: 21876760
Sci Rep. 2018 Nov 8;8(1):16550
pubmed: 30410112
Stem Cell Reports. 2020 Apr 14;14(4):663-676
pubmed: 32197114
Regen Med. 2009 Mar;4(2):225-37
pubmed: 19317642
Angew Chem Int Ed Engl. 2019 Jul 29;58(31):10563-10566
pubmed: 31166057
Stem Cells. 2009 Dec;27(12):2928-40
pubmed: 19725119
Biomaterials. 2020 Sep;253:120060
pubmed: 32450407
Regen Med. 2011 Jan;6(1):53-66
pubmed: 21175287
Nat Biotechnol. 2007 Sep;25(9):1015-24
pubmed: 17721512
Stem Cells. 2020 Mar;38(3):390-394
pubmed: 31778240
Biotechnol J. 2010 Jan;5(1):50-61
pubmed: 19946874
Cell Rep. 2018 Nov 13;25(7):1966-1981.e4
pubmed: 30428361
Nat Biotechnol. 2011 Oct 23;29(11):1011-8
pubmed: 22020386
Gastroenterology. 2007 Feb;132(2):720-32
pubmed: 17258722
Biotechnol Bioeng. 2012 Dec;109(12):3119-32
pubmed: 22674472
PLoS One. 2014 Jan 30;9(1):e88012
pubmed: 24498239
Nat Methods. 2013 May;10(5):438-44
pubmed: 23563795
Stem Cell Reports. 2016 Sep 13;7(3):543-556
pubmed: 27569060
Stem Cell Reports. 2018 Oct 9;11(4):852-860
pubmed: 30197117
Heliyon. 2020 Mar 03;6(3):e03493
pubmed: 32154424
Cell Res. 2011 Nov;21(11):1551-63
pubmed: 21894191
Stem Cell Res. 2017 Apr;20:58-66
pubmed: 28395742
Stem Cells. 2006 Jun;24(6):1450-7
pubmed: 16456125
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):E1848-57
pubmed: 22645348
Nat Biotechnol. 2011 Aug 14;29(9):829-34
pubmed: 21841799