Generation of NK cells with chimeric-switch receptors to overcome PD1-mediated inhibition in cancer immunotherapy.

Antibody-dependent cellular cytotoxicity Chimeric switch receptor Hematologic neoplasms Immunotherapy Natural killer cells Programmed cell death 1 receptor

Journal

Cancer immunology, immunotherapy : CII
ISSN: 1432-0851
Titre abrégé: Cancer Immunol Immunother
Pays: Germany
ID NLM: 8605732

Informations de publication

Date de publication:
May 2023
Historique:
received: 11 04 2022
accepted: 25 10 2022
medline: 19 4 2023
pubmed: 11 11 2022
entrez: 10 11 2022
Statut: ppublish

Résumé

Multiple myeloma (MM) is an incurable hematological cancer, in which immune checkpoint inhibition (ICI) with monoclonal antibodies (mAbs) has failed due to uncontrollable immune responses in combination therapies and lack of efficacy in monotherapies. Although NK cell-specific checkpoint targets such as NKG2A and KIRs are currently being evaluated in clinical trials, the clinical impact of NK cells on the PD1 cascade is less well understood compared to T cells. Furthermore, while NK cells have effector activity within the TME, under continuous ligand exposure, NK cell dysfunctionality may occur due to interaction of PD1 and its ligand PD-L1. Due to above-mentioned factors, we designed novel NK cell specific PD1-based chimeric switch receptors (PD1-CSR) by employing signaling domains of DAP10, DAP12 and CD3ζ to revert NK cell inhibition and retarget ICI. PD1-CSR modified NK cells showed increased degranulation, cytokine secretion and cytotoxicity upon recognition of PD-L1

Identifiants

pubmed: 36355079
doi: 10.1007/s00262-022-03317-y
pii: 10.1007/s00262-022-03317-y
pmc: PMC10110653
doi:

Substances chimiques

B7-H1 Antigen 0
Ligands 0
Cytokines 0
Receptors, Natural Killer Cell 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1153-1167

Subventions

Organisme : VINNOVA
ID : 2019-00056
Organisme : Radiumhemmets Forskningsfonder
ID : 191063

Informations de copyright

© 2022. The Author(s).

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Auteurs

Katharina H Susek (KH)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Ysabel A Schwietzer (YA)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Maria Karvouni (M)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Mari Gilljam (M)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Marton Keszei (M)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Alamdar Hussain (A)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Johan Lund (J)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Muhammad Kashif (M)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Andreas Lundqvist (A)

Department of Oncology-Pathology, Karolinska Institutet, Solna, Stockholm, Sweden.

Hans-Gustaf Ljunggren (HG)

Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Hareth Nahi (H)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Arnika K Wagner (AK)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Evren Alici (E)

Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden. evren.alici@ki.se.

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Classifications MeSH