Viral Gene Delivery in Chondrocytes.

Chondrocytes / physiology Transduction, Genetic Genetic Vectors / genetics Cartilage, Articular Gene Transfer Techniques Dependovirus / genetics Genetic Therapy / methods Genes, Viral
AAV Adenovirus Cartilage Lentivirus Retrovirus

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 10 11 2022
pubmed: 11 11 2022
medline: 15 11 2022
Statut: ppublish

Résumé

Viral gene transfer, known as transduction, is a powerful research tool for studying the biology of chondrocytes in novel ways and also a technology enabling the use of gene therapy for regenerating cartilage and treating diseases that affect cartilage, such as osteoarthritis. Adenovirus, retrovirus, lentivirus, and adeno-associated virus (AAV) are most commonly used to transduce chondrocytes. Although AAV is able to transduce chondrocytes in situ by intra-articular injection, chondrocytes are most commonly transduced in monolayer culture using the four vectors mentioned above. Protocols for achieving this are described, along with a discussion of the variables that can influence transduction efficiency.

Identifiants

DOI: 10.1007/978-1-0716-2839-3_20 PMID: 36355299
pubmed: 36355299
doi: 10.1007/978-1-0716-2839-3_20
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

289-300

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Evans CH, Ghivizzani SC, Smith P et al (2000) Using gene therapy to protect and restore cartilage. Clin Orthop Relat Res (379 Suppl):S214–S219. https://doi.org/10.1097/00003086-200010001-00027
Kantor A, McClements ME, MacLaren RE (2020) CRISPR-Cas9 DNA base-editing and prime-editing. Int J Mol Sci 21(17). https://doi.org/10.3390/ijms21176240
Lundstrom K (2018) Viral vectors in gene therapy. Diseases 6(2). https://doi.org/10.3390/diseases6020042
Evans CH (2019) The vicissitudes of gene therapy. Bone Joint Res 8(10):469–471. https://doi.org/10.1302/2046-3758.810.BJR-2019-0265
doi: 10.1302/2046-3758.810.BJR-2019-0265 pubmed: 31728185 pmcid: 6825047
Evans CH, Ghivizzani SC, Robbin PD (2021) Orthopaedic gene therapy: 25 years on. J Bone Joint Surg 9(8):e20.00220
Greber UF, Gomez-Gonzalez A (2021) Adenovirus – a blueprint for gene delivery. Curr Opin Virol 48:49–56. https://doi.org/10.1016/j.coviro.2021.03.006
doi: 10.1016/j.coviro.2021.03.006 pubmed: 33892224
Yang Y, Nunes FA, Berencsi K et al (1994) Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. Proc Natl Acad Sci U S A 91(10):4407–4411. https://doi.org/10.1073/pnas.91.10.4407
doi: 10.1073/pnas.91.10.4407 pubmed: 8183921 pmcid: 43794
Ricobaraza A, Gonzalez-Aparicio M, Mora-Jimenez L et al (2020) High-capacity adenoviral vectors: expanding the scope of gene therapy. Int J Mol Sci 21(10). https://doi.org/10.3390/ijms21103643
Ito T, Tokunaga K, Maruyama H et al (2003) Coxsackievirus and adenovirus receptor (CAR)-positive immature osteoblasts as targets of adenovirus-mediated gene transfer for fracture healing. Gene Ther 10(18):1623–1628. https://doi.org/10.1038/sj.gt.3302060
doi: 10.1038/sj.gt.3302060 pubmed: 12907955
Brower-Toland BD, Saxer RA, Goodrich LR et al (2001) Direct adenovirus-mediated insulin-like growth factor I gene transfer enhances transplant chondrocyte function. Hum Gene Ther 12(2):117–129. https://doi.org/10.1089/104303401750061186
doi: 10.1089/104303401750061186 pubmed: 11177549
Palmer GD, Gouze E, Gouze JN et al (2003) Gene transfer to articular chondrocytes with recombinant adenovirus. Methods Mol Biol 215:235–246. https://doi.org/10.1385/1-59259-345-3:235
doi: 10.1385/1-59259-345-3:235 pubmed: 12512303
Shuler FD, Georgescu HI, Niyibizi C et al (2000) Increased matrix synthesis following adenoviral transfer of a transforming growth factor beta1 gene into articular chondrocytes. J Orthop Res 18(4):585–592. https://doi.org/10.1002/jor.1100180411
doi: 10.1002/jor.1100180411 pubmed: 11052495
Smith P, Shuler FD, Georgescu HI et al (2000) Genetic enhancement of matrix synthesis by articular chondrocytes: comparison of different growth factor genes in the presence and absence of interleukin-1. Arthritis Rheum 43(5):1156–1164. https://doi.org/10.1002/1529-0131(200005)43:5<1156::AID-ANR26>3.0.CO;2-M
doi: 10.1002/1529-0131(200005)43:5<1156::AID-ANR26>3.0.CO;2-M pubmed: 10817570
Oberholzer A, John T, Kohl B et al (2007) Adenoviral transduction is more efficient in alginate-derived chondrocytes than in monolayer chondrocytes. Cell Tissue Res 328(2):383–390. https://doi.org/10.1007/s00441-006-0371-5
doi: 10.1007/s00441-006-0371-5 pubmed: 17265065
Watson Levings RS, Broome TA, Smith AD et al (2018) Gene therapy for osteoarthritis: pharmacokinetics of intra-articular self-complementary adeno-associated virus interleukin-1 receptor antagonist delivery in an equine model. Hum Gene Ther Clin Dev 29(2):90–100. https://doi.org/10.1089/humc.2017.142
doi: 10.1089/humc.2017.142 pubmed: 29869540 pmcid: 6007808
Yang J, Zhou W, Zhang Y et al (1999) Concatamerization of adeno-associated virus circular genomes occurs through intermolecular recombination. J Virol 73(11):9468–9477. https://doi.org/10.1128/JVI.73.11.9468-9477.1999
doi: 10.1128/JVI.73.11.9468-9477.1999 pubmed: 10516055 pmcid: 112981
Lisowski L, Tay SS, Alexander IE (2015) Adeno-associated virus serotypes for gene therapeutics. Curr Opin Pharmacol 24:59–67. https://doi.org/10.1016/j.coph.2015.07.006
doi: 10.1016/j.coph.2015.07.006 pubmed: 26291407
Chen Q, Luo H, Zhou C et al (2020) Comparative intra-articular gene transfer of seven adeno-associated virus serotypes reveals that AAV2 mediates the most efficient transduction to mouse arthritic chondrocytes. PLoS One 15(12):e0243359. https://doi.org/10.1371/journal.pone.0243359
doi: 10.1371/journal.pone.0243359 pubmed: 33320893 pmcid: 7737971
Goodrich LR, Choi VW, Carbone BA et al (2009) Ex vivo serotype-specific transduction of equine joint tissue by self-complementary adeno-associated viral vectors. Hum Gene Ther 20(12):1697–1702. https://doi.org/10.1089/hum.2009.030
doi: 10.1089/hum.2009.030 pubmed: 19642864 pmcid: 2861962
McCarty DM (2008) Self-complementary AAV vectors; advances and applications. Mol Ther 16(10):1648–1656. https://doi.org/10.1038/mt.2008.171
doi: 10.1038/mt.2008.171 pubmed: 18682697
Grimm D (2002) Production methods for gene transfer vectors based on adeno-associated virus serotypes. Methods 28(2):146–157. https://doi.org/10.1016/s1046-2023(02)00219-0
doi: 10.1016/s1046-2023(02)00219-0 pubmed: 12413413
Srivastava A, Mallela KMG, Deorkar N et al (2021) Manufacturing challenges and rational formulation development for AAV viral vectors. J Pharm Sci. https://doi.org/10.1016/j.xphs.2021.03.024
Kang R, Marui T, Ghivizzani SC et al (1997) Ex vivo gene transfer to chondrocytes in full-thickness articular cartilage defects: a feasibility study. Osteoarthr Cartil 5(2):139–143. https://doi.org/10.1016/s1063-4584(97)80007-6
doi: 10.1016/s1063-4584(97)80007-6
Maetzig T, Galla M, Baum C et al (2011) Gammaretroviral vectors: biology, technology and application. Viruses 3(6):677–713. https://doi.org/10.3390/v3060677
doi: 10.3390/v3060677 pubmed: 21994751 pmcid: 3185771
Milone MC, O’Doherty U (2018) Clinical use of lentiviral vectors. Leukemia 32(7):1529–1541. https://doi.org/10.1038/s41375-018-0106-0
doi: 10.1038/s41375-018-0106-0 pubmed: 29654266 pmcid: 6035154
Fouletier-Dilling CM, Bosch P, Davis AR et al (2005) Novel compound enables high-level adenovirus transduction in the absence of an adenovirus-specific receptor. Hum Gene Ther 16(11):1287–1297. https://doi.org/10.1089/hum.2005.16.1287
doi: 10.1089/hum.2005.16.1287 pubmed: 16259562
Palmer GD, Stoddart MJ, Gouze E et al (2008) A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors. Gene Ther 15(5):357–363. https://doi.org/10.1038/sj.gt.3303092
doi: 10.1038/sj.gt.3303092 pubmed: 18283289
Sapet C, Pellegrino C, Laurent N et al (2012) Magnetic nanoparticles enhance adenovirus transduction in vitro and in vivo. Pharm Res 29(5):1203–1218. https://doi.org/10.1007/s11095-011-0629-9
doi: 10.1007/s11095-011-0629-9 pubmed: 22146803
Del Vecchio MA, Georgescu HI, McCormack JE et al (2001) Approaches to enhancing the retroviral transduction of human synoviocytes. Arthritis Res 3(4):259–263. https://doi.org/10.1186/ar311
doi: 10.1186/ar311 pubmed: 11438045 pmcid: 34116

Auteurs

Christopher V Nagelli (CV)

Musculoskeletal Gene Therapy Laboratory, Mayo Clinic, Rochester, MN, USA.

Christopher H Evans (CH)

Musculoskeletal Gene Therapy Laboratory, Mayo Clinic, Rochester, MN, USA. Evans.Christopher@Mayo.edu.
Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN, USA. Evans.Christopher@Mayo.edu.

Rodolfo E De La Vega (RE)

Musculoskeletal Gene Therapy Laboratory, Mayo Clinic, Rochester, MN, USA.

Articles similaires

Hemiarthroplasty in young patients.

Hazimah Mahmud, Dong Wang, Andra Topan-Rat et al.
1.00
Humans Male Hemiarthroplasty Middle Aged Aged

A novel small molecule screening assay using normal human chondrocytes toward osteoarthritis drug discovery.

Philip R Coryell, Paul B Hardy, Susan Chubinskaya et al.
1.00
Humans Chondrocytes Osteoarthritis Matrix Metalloproteinase 13 Drug Discovery

Single cell analysis of Idh mutant growth plates identifies cell populations responsible for longitudinal bone growth and enchondroma formation.

Vijitha Puviindran, Eijiro Shimada, Zeyu Huang et al.
1.00
Animals Growth Plate Isocitrate Dehydrogenase Mice Single-Cell Analysis

Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina.

Satheesh Kumar, Yi-Wen Hsiao, Vickie H Y Wong et al.
1.00
Animals Humans CRISPR-Cas Systems Mice Vascular Endothelial Growth Factor A

Classifications MeSH

questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Chondrocytes › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Transduction génétique › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Appareil locomoteur › Squelette › Articulations › Cartilage articulaire › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Virus › Virus à ADN › Parvoviridae › Parvovirinae › Dependovirus › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Thérapie génétique › Viral Gene Delivery in Chondrocytes.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › Gènes viraux › Viral Gene Delivery in Chondrocytes.