The Gene Expression Landscape of Prostate Cancer BM Reveals Close Interaction with the Bone Microenvironment.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
27 Oct 2022
Historique:
received: 12 09 2022
revised: 12 10 2022
accepted: 23 10 2022
entrez: 11 11 2022
pubmed: 12 11 2022
medline: 15 11 2022
Statut: epublish

Résumé

Bone metastatic (BM) prostate cancer (PCa) belongs to the most lethal form of PCa, and therapeutic options are limited. Molecular profiling of metastases contributes to the understanding of mechanisms defining the bone metastatic niche. Our aim was to explore the transcriptional profile of PCa BM and to identify genes that drive progression. Paraffin-embedded tissues of 28 primary PCa and 30 BM were submitted to RNA extraction and analyzed by RNA sequencing using the Nanostring nCounter gene expression platform. A total of 770 cancer-related genes were measured using the Nanostring™ PanCancer progression panel. Gene Ontology (GO), KEGG, Reactome, STRING, Metascape, PANTHER, and Pubmed were used for data integration and gene annotation. We identified 116 differentially expressed genes (DEG) in BM compared to primaries. The most significant DEGs include

Identifiants

pubmed: 36361816
pii: ijms232113029
doi: 10.3390/ijms232113029
pmc: PMC9656691
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : SPP2084

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Auteurs

Alireza Saraji (A)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Kang Duan (K)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Christian Watermann (C)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Katharina Hempel (K)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Marie C Roesch (MC)

Department of Urology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Rosemarie Krupar (R)

Research Center Borstel, Leibniz Lung Center, 23845 Borstel, Germany.

Janine Stegmann-Frehse (J)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Danny Jonigk (D)

Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.

Mark Philipp Kuehnel (MP)

Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.

Wolfram Klapper (W)

Institute of Pathology, Hematopathology Section and Lymph Node Registry, Universitätsklinikum Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany.

Axel S Merseburger (AS)

Department of Urology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Jutta Kirfel (J)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Sven Perner (S)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.
Research Center Borstel, Leibniz Lung Center, 23845 Borstel, Germany.

Anne Offermann (A)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

Verena Sailer (V)

Institute of Pathology, University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.

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Classifications MeSH