Recognition of a Clickable Abasic Site Analog by DNA Polymerases and DNA Repair Enzymes.
AP endonucleases
AP site
DNA glycosylases
DNA polymerases
DNA repair
click chemistry
translesion synthesis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
01 Nov 2022
01 Nov 2022
Historique:
received:
08
10
2022
revised:
27
10
2022
accepted:
30
10
2022
entrez:
11
11
2022
pubmed:
12
11
2022
medline:
15
11
2022
Statut:
epublish
Résumé
Azide-alkyne cycloaddition ("click chemistry") has found wide use in the analysis of molecular interactions in living cells. 5-ethynyl-2-(hydroxymethyl)tetrahydrofuran-3-ol (EAP) is a recently developed apurinic/apyrimidinic (AP) site analog functionalized with an ethynyl moiety, which can be introduced into cells in DNA constructs to perform labeling or cross-linking in situ. However, as a non-natural nucleoside, EAP could be subject to removal by DNA repair and misreading by DNA polymerases. Here, we investigate the interaction of this clickable AP site analog with DNA polymerases and base excision repair enzymes. Similarly to the natural AP site, EAP was non-instructive and followed the "A-rule", directing residual but easily detectable incorporation of dAMP by
Identifiants
pubmed: 36362137
pii: ijms232113353
doi: 10.3390/ijms232113353
pmc: PMC9655677
pii:
doi:
Substances chimiques
DNA-(Apurinic or Apyrimidinic Site) Lyase
EC 4.2.99.18
DNA-Directed DNA Polymerase
EC 2.7.7.7
DNA Polymerase I
EC 2.7.7.7
Endonucleases
EC 3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Science Foundation
ID : 21-74-10104
Organisme : Russian Foundation for Basic Research
ID : 20-34-90092
Organisme : Russian Ministry of Science and Higher Education
ID : 121031300056-8
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