Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study.
MBL
haplogenotype
haplotype
polymorphism
recurrent aphthous stomatitis
Journal
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
ISSN: 1600-0714
Titre abrégé: J Oral Pathol Med
Pays: Denmark
ID NLM: 8911934
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
revised:
08
09
2022
received:
23
05
2022
accepted:
09
11
2022
pubmed:
14
11
2022
medline:
10
1
2023
entrez:
13
11
2022
Statut:
ppublish
Résumé
Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case-control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose-binding lectin 2 gene might affect the mannose-binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose-binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population. The study included 227 subjects; 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose-binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose-binding lectin serum levels were determined by enzyme-linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87). No significant differences in mean of mannose-binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml ± 249 standard deviation (SD) vs. 316 ng/ml ± 177 SD in remission phase vs. 343 ng/ml ± 254 SD in active phase; p > 0.05), also the allele and genotype frequencies of the studied mannose-binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75-1.23). Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1.23). This study did not confirm the previously reported association of the mannose-binding lectin 2 Gly54Asp gene variant and low mannose-binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose-binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.
Sections du résumé
BACKGROUND
BACKGROUND
Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case-control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose-binding lectin 2 gene might affect the mannose-binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose-binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population.
METHODS
METHODS
The study included 227 subjects; 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose-binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose-binding lectin serum levels were determined by enzyme-linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87).
RESULTS
RESULTS
No significant differences in mean of mannose-binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml ± 249 standard deviation (SD) vs. 316 ng/ml ± 177 SD in remission phase vs. 343 ng/ml ± 254 SD in active phase; p > 0.05), also the allele and genotype frequencies of the studied mannose-binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75-1.23). Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1.23).
CONCLUSIONS
CONCLUSIONS
This study did not confirm the previously reported association of the mannose-binding lectin 2 Gly54Asp gene variant and low mannose-binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose-binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.
Substances chimiques
Mannose-Binding Lectin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-90Subventions
Organisme : The Ministry of Health of the Czech Republic
ID : AZV15-29336A
Organisme : CETOCOEN EXCELLENCE
ID : CZ.02.1.01/0.0/0.0/17_043/0009632
Organisme : European Union´s Horizon 2020 Research and Innovation Programme: CETOCOEN Excellence Teaming Phase II
ID : 857560
Organisme : Masaryk University
ID : MUNI/A/1445/2021
Organisme : RECETOX RI
ID : LM2018121
Organisme : Czech Ministry of Education, Youth and Sports
Informations de copyright
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Wang Z, Cao H, Xiong J, et al. Recent advances in the aetiology of recurrent aphthous stomatitis (RAS). Postgrad Med J. 2022;98:57-66.
Baioumy SA, Fouad SH, Abdalgeleel SA, Baiomy AA, Sallam DE, Taha SI. Mannose-binding lectin serum levels and (Gly54Asp) gene polymorphism in recurrent aphthous stomatitis: a case-control study. Int J Immunopathol Pharmacol. 2021;35:20587384211064454.
Garred P, Genster N, Pilely K, et al. A journey through the lectin pathway of complement-MBL and beyond. Immunol Rev. 2016;274:74-97.
Zhou J, Hu M, Li J, et al. Mannan-binding lectin regulates inflammatory cytokine production, proliferation, and cytotoxicity of human peripheral natural killer cells. Mediators Inflamm. 2019;2019:6738286.
Uemura K, Yamamoto H, Nakagawa T, et al. Superoxide production from human polymorphonuclear leukocytes by human mannan-binding protein (MBP). Glycoconj J. 2004;21:79-84.
Sastry K, Herman GA, Day L, et al. The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10. J Exp Med. 1989;170:1175-1189.
Madsen HO, Garred P, Kurtzhals JA, et al. A new frequent allele is the missing link in the structural polymorphism of the human mannan binding protein. Immunogenetics. 1994;40:37-44.
Madsen HO, Garred P, Thiel S, et al. Interplay between promoter and structural gene variants control basal serum level of mannan-binding protein. J Immunol. 1995;155:3013-3020.
Madsen HO, Satz ML, Hogh B, Svejgaard A, Garred P. Different molecular events result in low protein levels of mannan-binding lectin in populations from southeast Africa and South America. J Immunol. 1998;161:3169-3175.
Lipscombe RJ, Sumiya M, Hill AV, et al. High frequencies in African and non-African populations of independent mutations in the mannose binding protein gene. Hum Mol Genet. 1992;1:709-715.
Lipscombe RJ, Beatty DW, Ganczakowski M, et al. Mutations in the human mannose-binding protein gene: frequencies in several population groups. Eur J Hum Genet. 1996;4:13-19.
Verdu P, Barreiro LB, Patin E, et al. Evolutionary insights into the high worldwide prevalence of MBL2 deficiency alleles. Hum Mol Genet. 2006;15:2650-2658.
Boldt AB, Messias-Reason IJ, Meyer D, et al. Phylogenetic nomenclature and evolution of mannose-binding lectin (MBL2) haplotypes. BMC Genet. 2010;14:1-12.
Karakus N, Yigit S, Rustemoglu A, Kalkan G, Bozkurt N. Effects of interleukin (IL)-6 gene polymorphisms on recurrent aphthous stomatitis. Arch Dermatol Res. 2014;306:173-180.
Slezakova S, Borilova Linhartova P, Masopustova L, et al. Association of the NOD-like receptor 3 (NLRP3) gene variability with recurrent aphthous stomatitis in the Czech population. J Oral Pathol Med. 2018;47:434-439.
Sambrook J, Fritsch E, Maniatis T. Molecular Cloning: A Laboratory Manual: Volume 2. 2nd ed. Cold Spring Harbor Laboratory Press; 1989.
Mrazkova J, Sistek P, Lochman J, Izakovicova Holla L, Danek Z, Borilova LP. A SNaPshot assay for determination of the mannose-binding lectin gene variants and an algorithm for calculation of haplogenotype combinations. Diagnostics. 2021;11:301.
Miller MF, Garfunkel AA, Ramp CA, Ship II. The inheritance of recurrent aphthous stomatitis. Observations on susceptibility. Oral Surg Oral Med Oral Pathol. 1980;49:409-412.
Ip WK, Takahashi K, Ezekowitz RA, Stuart LM. Mannose-binding lectin and innate immunity. Immunol Rev. 2009;230:9-21.
Heitzender S, Seidel M, Forster-Waldl E, Heitger A. Mannan-binding lectin deficiency-good news, bad news, doesn't matter? Clin Immunol. 2012;143:22-38.
Gröndahl-Yli-Hannuksela K, Viander M, Mertsola J, He Q. Increased risk of pertussis in adult patients with mannose-binding lectin deficiency. APMIS. 2013;121(4):311-315.
Olszowski T, Adler G, Janiszewska-Olszowska J, Safranow K, Kaczmarczyk M. MBL2, MASP2, AMELX, and ENAM gene polymorphisms and dental caries in Polish children. Oral Dis. 2012;18:389-395.
Mokhtari MJ, Koohpeima F, Hashemi-Gorji F. Association of the risk of dental caries and polymorphism of MBL2 rs11003125 gene in Iranian adults. Caries Res. 2019;53:60-64.
Hu XP, Zhou HJ, Li ZQ, Song TZ, Zhu YY. Lack of association between lactoferrin (LTF) and mannose-binding lectin 2 (MBL2) gene polymorphism and dental carries susceptibility. Int Med Res. 2020;48:300060520943428.
Yang Y, Tan H, Deng B, et al. Genetic polymorphisms of C-type lectin receptors in Behcet's disease in a Chinese Han population. Sci Rep. 2017;7:5348.
Kim J, Im CH, Kang EH, et al. Mannose-binding lectin gene-2 polymorphisms and serum mannose-binding lectin levels in Behcet's disease. Clin Exp Rheumatol. 2009;27:S13-S17.
Lewkowicz N, Lewkowicz P, Kurnatowska A, et al. Innate immune system is implicated inrecurrent aphthous ulcer pathogenesis. J Oral Pathol Med. 2003;32:475-481.
Inanc N, Mumcu G, Birtas E, et al. Serum mannose-binding lectin levels are decreased in Behcet's disease and associated with disease severity. J Rheumatol. 2005;32:287-291.
Eisen DP, Osthoff M. If there is an evolutionary selection pressure for the high frequency of MBL polymorphisms, what is it? Clin Exp Immunol. 2014;176:165-171.
Cedzyński M, Kilpatrick DC, Świerzko AS. Mannose-binding lectin. In: Barnum S, Schein T, eds. The Complement FactsBook. Elsevier; 2018:33-43.