Characterizing microglial gene expression in a model of secondary progressive multiple sclerosis.
RNA sequencing
experimental autoimmune encephalitis
microglia
secondary progressive MS
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
revised:
30
09
2022
received:
16
03
2022
accepted:
26
10
2022
pubmed:
16
11
2022
medline:
25
1
2023
entrez:
15
11
2022
Statut:
ppublish
Résumé
Multiple sclerosis (MS) is the most common inflammatory, demyelinating and neurodegenerative disease of the central nervous system in young adults. Chronic-relapsing experimental autoimmune encephalomyelitis (crEAE) in Biozzi ABH mice is an experimental model of MS. This crEAE model is characterized by an acute phase with severe neurological disability, followed by remission of disease, relapse of neurological disease and remission that eventually results in a chronic progressive phase that mimics the secondary progressive phase (SPEAE) of MS. In both MS and SPEAE, the role of microglia is poorly defined. We used a crEAE model to characterize microglia in the different phases of crEAE phases using morphometric and RNA sequencing analyses. At the initial, acute inflammation phase, microglia acquired a pro-inflammatory phenotype. At the remission phase, expression of standard immune activation genes was decreased while expression of genes associated with lipid metabolism and tissue remodeling were increased. Chronic phase microglia partially regain inflammatory gene sets and increase expression of genes associated with proliferation. Together, the data presented here indicate that microglia obtain different features at different stages of crEAE and a particularly mixed phenotype in the chronic stage. Understanding the properties of microglia that are present at the chronic phase of EAE will help to understand the role of microglia in secondary progressive MS, to better aid the development of therapies for this phase of the disease.
Identifiants
pubmed: 36377669
doi: 10.1002/glia.24297
pmc: PMC10100411
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
588-601Subventions
Organisme : Italian MS Foundation
ID : 2011/B/7
Organisme : Stichting MS Research
ID : 10-723
Organisme : Stichting MS Research
ID : 16-947
Informations de copyright
© 2022 The Authors. GLIA published by Wiley Periodicals LLC.
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