A genotyping by sequencing approach can disclose Apis mellifera population genomic information contained in honey environmental DNA.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 11 2022
15 11 2022
Historique:
received:
30
07
2022
accepted:
10
11
2022
entrez:
15
11
2022
pubmed:
16
11
2022
medline:
19
11
2022
Statut:
epublish
Résumé
Awareness has been raised over the last years on the genetic integrity of autochthonous honey bee subspecies. Genomic tools available in Apis mellifera can make it possible to measure this information by targeting individual honey bee DNA. Honey contains DNA traces from all organisms that contributed or were involved in its production steps, including the honey bees of the colony. In this study, we designed and tested a genotyping by sequencing (GBS) assay to analyse single nucleotide polymorphisms (SNPs) of A. mellifera nuclear genome using environmental DNA extracted from honey. A total of 121 SNPs (97 SNPs informative for honey bee subspecies identification and 24 SNPs associated with relevant traits of the colonies) were used in the assay to genotype honey DNA, which derives from thousands of honey bees. Results were integrated with information derived from previous studies and whole genome resequencing datasets. This GBS method is highly reliable in estimating honey bee SNP allele frequencies of the whole colony from which the honey derived. This assay can be used to identify the honey bee subspecies of the colony that produced the honey and, in turn, to authenticate the entomological origin of the honey.
Identifiants
pubmed: 36379985
doi: 10.1038/s41598-022-24101-z
pii: 10.1038/s41598-022-24101-z
pmc: PMC9666642
doi:
Substances chimiques
DNA, Environmental
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19541Subventions
Organisme : Università di Bologna
ID : 2020-2021 RFO program
Organisme : Regione Emilia-Romagna
ID : BEE-RER-3 projects - CUP E37G22000030007 - del Regolamento (UE) no. 1308/2013 - (OCM Apicoltura)
Organisme : EGI-ACE
ID : 101017567
Informations de copyright
© 2022. The Author(s).
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