Tridimensional cell culture of dermal fibroblasts promotes exosome-mediated secretion of extracellular matrix proteins.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 11 2022
17 11 2022
Historique:
received:
08
03
2022
accepted:
31
10
2022
entrez:
17
11
2022
pubmed:
18
11
2022
medline:
22
11
2022
Statut:
epublish
Résumé
Extracellular matrix (ECM) secretion, deposition and assembly are part of a whole complex biological process influencing the microenvironment and other cellular behaviors. Emerging evidence is attributing a significant role to extracellular vesicles (EVs) and exosomes in a plethora of ECM-associated functions, but the role of dermal fibroblast-derived EVs in paracrine signalling is yet unclear. Herein, we investigated the effect of exosomes isolated from stimulated human dermal fibroblasts. We report that tridimensional (3D) cell culture of dermal fibroblasts promotes secretion of exosomes carrying a large quantity of proteins involved in the formation, organisation and remodelling of the ECM. In our 3D model, gene expression was highly modulated and linked to ECM, cellular migration and proliferation, as well as inflammatory response. Mass spectrometry analysis of exosomal proteins, isolated from 3D cultured fibroblast-conditioned media, revealed ECM protein enrichment, of which many were associated with the matrisome. We also show that the cytokine interleukin 6 (IL-6) is predicted to be central to the signalling pathways related to ECM formation and contributing to cell migration and proliferation. Overall, our data suggest that dermal fibroblast-derived EVs participate in many steps of the establishment of dermis's ECM.
Identifiants
pubmed: 36396670
doi: 10.1038/s41598-022-23433-0
pii: 10.1038/s41598-022-23433-0
pmc: PMC9672399
doi:
Substances chimiques
Extracellular Matrix Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19786Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2022. The Author(s).
Références
Sci Rep. 2021 Mar 23;11(1):6655
pubmed: 33758206
Biochim Biophys Acta Mol Cell Res. 2017 Nov;1864(11 Pt A):1989-2000
pubmed: 28578911
J Invest Dermatol. 1991 Oct;97(4):686-92
pubmed: 1940439
J Immunol Res. 2014;2014:964069
pubmed: 25126586
Sci Rep. 2019 Sep 10;9(1):13012
pubmed: 31506601
Sci Rep. 2016 Sep 12;6:32993
pubmed: 27615560
Biotechnol J. 2021 Jun;16(6):e2000250
pubmed: 33689228
Lupus. 2004;13(5):339-43
pubmed: 15230289
Cell Biosci. 2019 Feb 15;9:19
pubmed: 30815248
J Investig Dermatol Symp Proc. 2000 Dec;5(1):40-6
pubmed: 11147674
Nat Rev Immunol. 2014 Mar;14(3):195-208
pubmed: 24566916
Biomed Res Int. 2020 Apr 13;2020:6051210
pubmed: 32352002
J Neurochem. 2002 Nov;83(4):885-94
pubmed: 12421361
J Extracell Vesicles. 2019 Apr 01;8(1):1596016
pubmed: 30988894
Biochim Biophys Acta. 2016 Nov;1863(11):2795-2808
pubmed: 27599715
J Anat. 2015 Dec;227(6):746-56
pubmed: 25411113
J Glaucoma. 2014 Oct-Nov;23(8 Suppl 1):S20-3
pubmed: 25275899
Front Mol Biosci. 2020 Mar 06;7:33
pubmed: 32211418
Acta Biomater. 2015 Jan;11:3-16
pubmed: 25266503
Cell. 1982 Aug;30(1):215-24
pubmed: 7127471
Cells. 2018 Oct 13;7(10):
pubmed: 30322133
Int J Mol Sci. 2018 Oct 04;19(10):
pubmed: 30287763
J Immunol. 2009 Sep 1;183(5):3170-6
pubmed: 19648274
Cell Commun Signal. 2020 Nov 4;18(1):177
pubmed: 33148271
J Cell Biochem. 2012 Nov;113(11):3363-70
pubmed: 22644871
Minerva Med. 2021 Jun;112(3):384-400
pubmed: 33263376
J Cell Sci. 2012 Jul 1;125(Pt 13):3015-24
pubmed: 22797912
Adv Drug Deliv Rev. 2016 Feb 1;97:4-27
pubmed: 26562801
Semin Cell Dev Biol. 2017 Nov;71:99-105
pubmed: 28630027
Neural Regen Res. 2014 Jun 1;9(11):1154-62
pubmed: 25206775
Front Cell Dev Biol. 2018 Feb 20;6:18
pubmed: 29515996
Stem Cell Res Ther. 2017 Aug 14;8(1):189
pubmed: 28807034
Biomedicines. 2020 Apr 30;8(5):
pubmed: 32365896
Acc Chem Res. 2017 Apr 18;50(4):703-713
pubmed: 28345876
Eur Surg Res. 2017;58(1-2):81-94
pubmed: 27974711
Sci Rep. 2020 Oct 14;10(1):17300
pubmed: 33057073
Mod Rheumatol. 2019 Mar;29(2):294-301
pubmed: 30561237
Physiology (Bethesda). 2017 Jul;32(4):266-277
pubmed: 28615311
Cells. 2019 Jul 15;8(7):
pubmed: 31311206
Cancer Res. 2018 Sep 1;78(17):4957-4970
pubmed: 29976575
Cell Death Dis. 2018 Mar 7;9(3):382
pubmed: 29515112
Cancers (Basel). 2019 Jan 17;11(1):
pubmed: 30658425
Nat Rev Clin Oncol. 2018 Apr;15(4):234-248
pubmed: 29405201
Proteomics. 2013 May;13(10-11):1624-36
pubmed: 23526769
Proteomics. 2015 Jan;15(2-3):260-71
pubmed: 25307053
Acta Trop. 2019 Aug;196:30-36
pubmed: 31071298
Matrix Biol. 2019 Jan;75-76:201-219
pubmed: 29066152
Sci Adv. 2016 Jun 10;2(6):e1600502
pubmed: 27386584
Sci Rep. 2018 Jun 12;8(1):8973
pubmed: 29895824
Nat Commun. 2020 Oct 9;11(1):5120
pubmed: 33037194
Pharmacol Rev. 2012 Jul;64(3):676-705
pubmed: 22722893
Int J Mol Sci. 2020 Jun 25;21(12):
pubmed: 32630531
Mol Cancer Res. 2018 Jul;16(7):1196-1204
pubmed: 29636362
Biochim Biophys Acta. 2015 Nov;1852(11):2362-71
pubmed: 26300484
Front Immunol. 2021 Apr 26;12:671485
pubmed: 33981316
Clinics (Sao Paulo). 2020 Oct 19;75:e1801
pubmed: 33084768
Int J Legal Med. 2000;113(3):140-5
pubmed: 10876984
Adv Exp Med Biol. 2018;1119:119-131
pubmed: 30051320
NPJ Breast Cancer. 2018 Jul 16;4:18
pubmed: 30038960
Microsc Res Tech. 2003 Jan 1;60(1):107-14
pubmed: 12500267
Lab Invest. 1994 Feb;70(2):176-82
pubmed: 8139259
Annu Rev Cell Dev Biol. 2014;30:255-89
pubmed: 25288114
Blood Rev. 2013 Jan;27(1):31-9
pubmed: 23261067
Matrix Biol. 2018 Jan;65:104-118
pubmed: 28888912
Nature. 2013 Dec 12;504(7479):277-281
pubmed: 24336287
Stem Cells Dev. 2021 Sep 15;30(18):922-933
pubmed: 34167333
Cell Mol Life Sci. 2011 Aug;68(16):2667-88
pubmed: 21560073
Proteomics. 2019 Apr;19(8):e1800149
pubmed: 30758141
Front Pharmacol. 2018 Sep 21;9:979
pubmed: 30298000
Acta Neuropathol Commun. 2015 Jan 31;3:5
pubmed: 25637145
Respir Res. 2015 Aug 20;16:99
pubmed: 26289430
J Cell Biol. 2012 Feb 20;196(4):395-406
pubmed: 22351925
Int J Mol Sci. 2018 Jun 09;19(6):
pubmed: 29890746
Nat Commun. 2011;2:282
pubmed: 21505438
Adv Sci (Weinh). 2020 Oct 27;7(23):2002596
pubmed: 33304765