An ancestral Wnt-Brachyury feedback loop in axial patterning and recruitment of mesoderm-determining target genes.
Journal
Nature ecology & evolution
ISSN: 2397-334X
Titre abrégé: Nat Ecol Evol
Pays: England
ID NLM: 101698577
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
received:
26
07
2021
accepted:
12
09
2022
pubmed:
19
11
2022
medline:
6
12
2022
entrez:
18
11
2022
Statut:
ppublish
Résumé
Transcription factors are crucial drivers of cellular differentiation during animal development and often share ancient evolutionary origins. The T-box transcription factor Brachyury plays a pivotal role as an early mesoderm determinant and neural repressor in vertebrates; yet, the ancestral function and key evolutionary transitions of the role of this transcription factor remain obscure. Here, we present a genome-wide target-gene screen using chromatin immunoprecipitation sequencing in the sea anemone Nematostella vectensis, an early branching non-bilaterian, and the sea urchin Strongylocentrotus purpuratus, a representative of the sister lineage of chordates. Our analysis reveals an ancestral gene regulatory feedback loop connecting Brachyury, FoxA and canonical Wnt signalling involved in axial patterning that predates the cnidarian-bilaterian split about 700 million years ago. Surprisingly, we also found that part of the gene regulatory network controlling the fate of neuromesodermal progenitors in vertebrates was already present in the common ancestor of cnidarians and bilaterians. However, while several endodermal and neuronal Brachyury target genes are ancestrally shared, hardly any of the key mesodermal downstream targets in vertebrates are found in the sea anemone or the sea urchin. Our study suggests that a limited number of target genes involved in mesoderm formation were newly acquired in the vertebrate lineage, leading to a dramatic shift in the function of this ancestral developmental regulator.
Identifiants
pubmed: 36396969
doi: 10.1038/s41559-022-01905-w
pii: 10.1038/s41559-022-01905-w
doi:
Substances chimiques
Brachyury protein
EQ43SC3GDB
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1921-1939Subventions
Organisme : Austrian Science Fund FWF
ID : P 30404
Pays : Austria
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Limited.
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