Illuminati: a form of gene expression plasticity in Drosophila neural stem cells.
Drosophila
Fluorescent reporters
Genome instability
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 11 2022
15 11 2022
Historique:
received:
29
03
2022
accepted:
06
10
2022
entrez:
18
11
2022
pubmed:
19
11
2022
medline:
23
11
2022
Statut:
ppublish
Résumé
While testing for genome instability in Drosophila as reported by unscheduled upregulation of UAS-GFP in cells that co-express GAL80 and GAL4, we noticed that, as expected, background levels were low in most developing tissues. However, GFP-positive clones were frequent in the larval brain. Most of these clones originated from central brain neural stem cells. Using imaging-based approaches and genome sequencing, we show that these unscheduled clones do not result from chromosome loss or mutations in GAL80. We have named this phenomenon 'Illuminati'. Illuminati is strongly enhanced in brat tumors and is also sensitive to environmental conditions such as food content and temperature. Illuminati is suppressed by Su(var)2-10, but it is not significantly affected by several modifiers of position effect variegation or Gal4::UAS variegation. We conclude that Illuminati identifies a previously unknown type of functional instability that may have important implications in development and disease.
Identifiants
pubmed: 36399062
pii: 282932
doi: 10.1242/dev.200808
pmc: PMC9845751
pii:
doi:
Substances chimiques
brat protein, Drosophila
0
DNA-Binding Proteins
0
Drosophila Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 2011 294603
Pays : International
Organisme : Marie Curie
Pays : United Kingdom
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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