Enhanced Antibacterial Activity of Highly Biocompatible Polymeric Core-Shell Levofloxacin Gold Nanocomposite Formulation Against Pseudomonas aeruginosa.
Chitosan
Core–shell nanocomposite
Gold nanoparticles
Levofloxacin
Polyvinyl alcohol
Pseudomonas aeruginosa
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
accepted:
08
11
2022
pubmed:
19
11
2022
medline:
17
2
2023
entrez:
18
11
2022
Statut:
ppublish
Résumé
Using natural and synthetic polymers as the components for the core-shell nanocomposite preparation has received recent attention in biomedicine due to their high biocompatibility, high efficacy, and biodegradability. In this present investigation, chitosan-polyvinyl alcohol core-shell gold nanocomposite was synthesised adopting green science principles followed by fabrication with fluoroquinolone antibiotic levofloxacin (LE-CS-PVA-AuNC). Core-shell nanocomposite was prepared from biogenic gold nanoparticles, chitosan, polyvinyl alcohol polymer mixture, and levofloxacin under optimum conditions, and the synthesised nanocomposite exhibited a highly stable nanoarchitecture. Enhancement of antibacterial activity of the nanocomposite was evaluated against the clinical strain of Pseudomonas aeruginosa by determination of growth inhibition, survival rate parameters, and biofilm inhibition rate. Levofloxacin-fabricated core-shell nanocomposite brought about higher growth inhibition than the free levofloxacin, which was confirmed by a notable zone of inhibition, growth inhibition at a lower concentration, rapid biofilm inhibitory rate, and changes in survival growth parameters. In vitro drug release pattern was studied by continuous dialysis, which reveals that the nanocomposite exhibited controlled, sustained release pattern and cumulative release reached almost 98.0% at 72 h. Biocompatibility was studied with human keratinocytes (HaCaT cell line), which was studied by measuring cell viability, oxidative stress marker protein, and genotoxicity. The tested nanocomposite was not inducing any sign of toxicity which was confirmed by no marked impact on cell viability, intracellular reduced glutathione, lipid peroxidase, and lactate dehydrogenase activity. In addition, the nanocomposite has not shown any toxic effect on DNA, and all findings indicate that the synthesised nanocomposite was compatible with human keratinocytes. LE-CS-PVA-AuNC synthesised in the present system adopting green science principles can be used in modern biomedicine as an effective and safe antimicrobial agent due to its high antimicrobial action against wound infection pathogens and its best compatibility with human keratinocytes.
Identifiants
pubmed: 36399305
doi: 10.1007/s12010-022-04256-1
pii: 10.1007/s12010-022-04256-1
doi:
Substances chimiques
Levofloxacin
6GNT3Y5LMF
Gold
7440-57-5
Chitosan
9012-76-4
Polyvinyl Alcohol
9002-89-5
Anti-Bacterial Agents
0
Polymers
0
Anti-Infective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1837-1861Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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