Research Note: A novel peptide-based ELISA for efficient detection of antibody against chicken infectious anemia virus.

Animals Chickens Chicken anemia virus Reproducibility of Results Poultry Diseases Enzyme-Linked Immunosorbent Assay / veterinary Antibodies, Viral Peptides
CIAV antibodies chicken infectious anaemia virus indirect ELISA peptide

Journal

Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150

Informations de publication

Date de publication:
Jan 2023
Historique:
received: 12 07 2022
revised: 13 10 2022
accepted: 19 10 2022
pubmed: 19 11 2022
medline: 10 1 2023
entrez: 18 11 2022
Statut: ppublish

Résumé

Chicken infectious anemia virus (CIAV) is the pathogen of chicken infectious anemia. Currently, due to the lack of effective diagnostics technology and prevention approach, CIAV has spread globally and caused huge economic losses to poultry industry. In this study, a novel peptide-based ELISA (pELISA) for efficient detection of antibody against CIAV was developed. The peptide (25CRLRRRYKFRHRRRQRYRRRAF45) used in pELISA was highly conserved in VP1 protein of different CIAV isolates. The specificity and reproducibility showed that the pELISA only reacted with sera against CIAV, not with sera against other pathogens tested, and the CV of the intra-/inter-assay of the pELISA was 6.8 to 9.22%. Moreover, the comparison assay using 56 clinical samples showed that the positive rate of the pELISA and the commercial ELISA kit (IDEXX) was 85.7 and 80.4%, respectively. The pELISA generated here provides a rapid and efficient serological detection method for diagnosis of CIAV infection and evaluation of the efficacy of CIAV vaccination.

Identifiants

DOI: 10.1016/j.psj.2022.102284 PMID: 36399931 PMC: PMC9673107
pubmed: 36399931
pii: S0032-5791(22)00579-X
doi: 10.1016/j.psj.2022.102284
pmc: PMC9673107
pii:
doi:

Substances chimiques

Antibodies, Viral 0
Peptides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102284

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Hongxia Shao (H)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Jinzhi Li (J)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Jun Zhang (J)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Qi Zhang (Q)

Wens Foodstuff Group Co., Ltd, Yunfu, Guangdong, China 527400.

Li Ma (L)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Jinhua Lu (J)

Wens Foodstuff Group Co., Ltd, Yunfu, Guangdong, China 527400.

Tuofan Li (T)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Quan Xie (Q)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Zhimin Wan (Z)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Aijian Qin (A)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009.

Jianqiang Ye (J)

Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China 225009; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China 225009; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China 225009; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China 225009. Electronic address: jqye@yzu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Virus › Virus à ADN › Circoviridae › Gyrovirus › Virus de l'anémie du poulet › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Maladies de l'animal › Maladies des oiseaux › Maladies de la volaille › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Research Note: A novel peptide-based ELISA for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Research Note: A novel peptide-based ELISA for...